 | Sigourney Weaver als Ripley 8 |
 | Winona Ryder als Annalee Call |
 | Dominique Pinon als Dom Vriess |
 | Ron Perlman als Ron Johner |
 | Gary Dourdan als Gary Christie |
 | Michael Wincott als Frank Elgyn |
 | Kim Flowers als Sabra Hillard |
 | Dan Hedaya als Gen. Martin Allahandro Carlos Perez |
 | J.E. Freeman als Dr. Mason Wren |
 | Brad Dourif als Dr. Jonathan Gediman |
 | Raymond Cruz als Pvt. Vincent Distephano |
 | Leland Orser als Larry Purvis |
 | Carolyn Campbell als Anesthesiologist |
| Marlene Bush als Scientist |
 | David St. James als Surgeon |
| Rodney Mitchell als Soldier with Glove |
| Robert Faltisco als Soldier Shot Through Helmet |
| David Rowe als Frozen Soldier |
| Garrett House als Soldier |
| Rod Damer als Soldier |
| Mark Mansfield als Soldier |
 | Daniel Raymont als Soldier |
 | Cris D'Annunzio als Soldier |
 | Steven Gilborn als Father (voice) |
| Robert Bastens als Sleeper |
 | Rico Bueno als Sleeper |
 | Alex Lorre als Sleeper |
| Ronald Ramessar als Sleeper |
| Nicole Fellows als Young Ripley |
 | Tom Woodruff Jr. als Lead Alien |
| Joan La Barbara als Newborn Vocal #1 (voice) |
 | Archie Hahn als Newborn Vocal #2 (voice) |
 | Eddie Yansick als Soldier Who Stuns Ripley |
 | Nito Larioza als Sleeper (uncredited) |
 | David Prior als Alien (uncredited) |
 | Nicholas Talone als Soldier in Cocoon (uncredited) |
| Brad Martin als Soldier Shot by Johner (uncredited) |
 | Wanda De Jesus als (uncredited) |
 | Jensen Ackles als Dead Soldier (uncredited) |
 | Alec Gillis als Hero Alien Warrior (uncredited) |
| John M. Dwyer | Set Decoration |
| David Giler | Producer |
| Walter Hill | Producer |
| Darius Khondji | Director of Photography |
| Jean-Pierre Jeunet | Director |
| Hervé Schneid | Editor |
| Bob Ringwood | Costume Design |
| Richard Pagano | Casting |
| Sigourney Weaver | Co-Producer |
| Pitof | Visual Effects Supervisor |
| Dan O'Bannon | Characters |
| Ronald Shusett | Characters |
| Gordon Carroll | Producer |
| H. R. Giger | Creature Design |
| Andrew Neskoromny | Art Direction |
| Katina Le Kerr | Costumer |
| Eric Allard | Special Effects Coordinator |
| Suzanne Tenner | Still Photographer |
| Nigel Phelps | Production Design |
| Chad Stahelski | Stunt Double |
| Joss Whedon | Screenplay |
| Harry B. Miller III | Dialogue Editor |
| Leslie Shatz | Sound Designer |
| John Frizzell | Original Music Composer |
| Maya Shimoguchi | Set Designer |
| Bill Badalato | Producer |
| Alan D'Angerio | Hair Designer |
| Conrad W. Hall | Camera Operator |
| Conrad W. Hall | VFX Director of Photography |
| Erik Henry | Visual Effects Supervisor |
| Alain Carsoux | Compositing Supervisor |
| R.J. Kizer | ADR Supervisor |
| Eyde Belasco | Casting Associate |
| Matthew Ferro | Visual Effects Producer |
| Adolfo Martínez Pérez | Storyboard Artist |
| Edward Conna | Stunts |
| Steve Wang | Sculptor |
| George Stokes | Propmaker |
| Peter Davidian | Rigging Gaffer |
| Ernie F. Orsatti | Stunt Coordinator |
| Brad Martin | Stunts |
| David Orr | Color Timer |
| Terri Douglas | ADR Voice Casting |
| Chris Cunningham | Character Technical Supervisor |
| Bill Boes | Assistant Art Director |
| Steve Cooper | Supervising Art Director |
| Mike Smithson | Makeup Artist |
| Mike Smithson | Sculptor |
| Claudia Wick | Costumer |
| David Rawley | Costume Supervisor |
| Jann K. Engel | Set Designer |
| John Chichester | Set Designer |
| Barry R. Koper | Makeup Department Head |
| Dan O'Connell | Foley |
| Gregory H. Watkins | Sound Re-Recording Mixer |
| Shannon Kenny | Script Supervisor |
| John T. Cucci | Foley |
| Ted Caplan | Foley Editor |
| Richard E. Hollander | Visual Effects Supervisor |
| Rick Kline | Sound Re-Recording Mixer |
| Chris O'Hara | Stunts |
| Luis G. Hoyos | Set Designer |
| Sean Garnhart | Sound Effects Editor |
| Jay Wilkinson | Sound Effects Editor |
| Dave Kulczycki | Dialogue Editor |
| Gary A. Krakoff | Construction Coordinator |
| Mick Cukurs | Set Designer |
| Nancy King | Second Assistant Director |
| Joe Viskocil | Pyrotechnic Supervisor |
| Emily Ferry | Property Master |
| Penny Smartt-Juday | Art Department Coordinator |
| William K. Dolan | Leadman |
| Richard Fernandez | Set Designer |
| Greg Sanger | Leadman |
| Steve Koch | Sculptor |
| Jeffrey P. Buccacio Jr. | Sculptor |
| John A. Larsen | Supervising Sound Editor |
| Linda Lew | Foley |
| Robin Haskins | Sound Recordist |
| David Cunningham | Sound Recordist |
| Martin November | Visual Effects Editor |
| Susan Zwerman | Visual Effects Producer |
| David Emmerichs | Steadicam Operator |
| Pete Romano | Underwater Director of Photography |
| Abby Treloggen | Music Editor |
| Dave Robling | Transportation Coordinator |
| Rachel Aberly | Unit Publicist |
| Rachel Aberly | Publicist |
| Carlos M. Torres | Electrician |
| Thomas Duval | Compositing Supervisor |
| Noon Orsatti | Stunts |
| Naomi Donne | Makeup Designer |
| Bill Myer | Makeup Artist |
| Gregory Zemgals | Set Dresser |
| Ed Fincher | Key Costumer |
| Paul Anthony Morris | Hairstylist |
| Antoine Durr | Digital Supervisor |
| Paul Urmson | Sound Effects Designer |
| Gregory Lynch Jr. | Carpenter |
| Robb Earnest | Production Secretary |
| Zoran Veselic | First Assistant Camera |
| Don Steinberg | Second Assistant Camera |
| James Andrykowski | First Assistant Editor |
| Terrence Harris | Casting Associate |
| Andy Friend | Storyboard Artist |
| Dana Kroeger | Camera Loader |
| Mike Defeo | Modeling |
| Michael Alexonis | Best Boy Grip |
| Michael Arvanitis | Best Boy Electric |
| Bob Leitelt | Key Rigging Grip |
| Cliff Berns | Painter |
| Mike Larrabee | Painter |
| Robert E. Denne | Painter |
| Thomas P. Powell | Lighting Technician |
| Gary Burritt | Negative Cutter |
| Chris Strong | Chief Lighting Technician |
| C. Alan Rawlins | Key Grip |
| Lauren Palmer | ADR Editor |
| Mark Cross | Music Producer |
| Charles S. Serrano | Propmaker |
| Susan Walter | Assistant Director |
| Steve Bowerman | Boom Operator |
| Jasen Joseph Sica | Key Hair Stylist |
| Mark Oppenheimer | First Assistant Director |
| Jon Marsala | Propmaker |
| Robert Hutchins | Pyrotechnician |
| C. Ashley Sudge | Dolly Grip |
| Liza Sullivan | Assistant Editor |
| Cynthia Quan | Production Accountant |
| Mitch Kopelman | Digital Effects Supervisor |
| Elijah Bryant | Set Dresser |
| Jerry Wax | Set Dresser |
| Paul Delucca | Costumer |
| Joy Taylor | Assistant Property Master |
| Bill Dance | Extras Casting |
| Peter McAdams | Grip |
| Gregg Sargeant | Stunt Double |
| Dean B. Katz | Set Dresser |
| Marc Martin Del Campo | Set Dresser |
| Mary Peters | Stunt Double |
| Chris Franco | Electrician |
| Gary S. Christie | Best Boy Grip |
| James Peattie | Construction Coordinator |
| James Hogue | Painter |
| Ernest von Hauff | Painter |
| Dan Brodzik | Painter |
| Joseph R. Pinkos | Set Dresser |
| John A. Mileski | Standby Painter |
| William T. McKane | Best Boy Electric |
| Dan Kerns | Electrician |
| Monty Woodard | Electrician |
| Michael Lambert | Electrician |
| Cathy Hill | Costumer |
| Jennifer Morrison Holyfield | Costumer |
| Lauren Smith | Visual Effects Coordinator |
| Barbara Crow | First Assistant Accountant |
| Ivan Martin del Campo | Production Secretary |
| Gene Kozicki | Production Manager |
| Bob Johnston | Production Supervisor |
| Jackie C. Lee | Second Second Assistant Director |
| John Dods | Makeup Artist |
