 | Zachary Levi als Toby |
 | David Cross als Ian |
 | Jason Lee als Dave |
 | Justin Long als Alvin (voice) |
 | Matthew Gray Gubler als Simon (voice) |
 | Jesse McCartney als Theodore (voice) |
 | Amy Poehler als Eleanor (voice) |
 | Anna Faris als Jeanette (voice) |
 | Christina Applegate als Brittany (voice) |
 | Wendie Malick als Dr. Rubin |
 | Anjelah Johnson-Reyes als Julie |
 | Kathryn Joosten als Aunt Jackie |
 | Kevin G. Schmidt als Ryan |
 | Chris Warren als Xander |
 | Bridgit Mendler als Becca |
 | Aimee Carrero als Emily |
 | Alexandra Shipp als Valentina |
 | Gregg Binkley als Emcee |
 | Jake Zyrus als Charice Pempengco |
 | Bernard White als Doctor |
 | Adele Jacques als Nurse |
 | Joy Osmanski als Airline Rep |
 | Archie Hahn als Agent |
 | Lanny Joon als Paramedic |
 | Brando Eaton als Jeremy |
 | Michael Bruno als Honor Society |
| Andrew Lee Schmidt als Honor Society |
| Alexander Noyes als Honor Society |
| Jason Rosen als Honor Society |
| Ali Mikles als Screaming Fan |
 | Eric Bauza als Digger the NASCAR Gopher (voice) |
 | Sean Astin als Meerkat Manor Narrator (voice) |
 | Marty Dew als Chipmunk Dancer |
| Richy Jackson als Chipmunk Dancer |
| Mihran Kirakosian als Chipmunk Dancer |
| Janelle Ginestra als Chipette Dancer |
 | Thomasina Gross als Chipette Dancer |
 | Rachele Brooke Smith als Chipette Dancer |
| Ryan Conferido als Li'l Rosero Dancer |
| Hokuto 'Hok' Konishi als Li'l Rosero Dancer |
| Ryan Feng als Li'l Rosero Dancer |
 | Dominic Sandoval als Li'l Rosero Dancer |
| Brian Hirano als Li'l Rosero Dancer |
 | Steve Terada als Li'l Rosero Dancer |
| Victor Kim als Li'l Rosero Dancer |
| Brittany Anne Pirtle als Cheerleader (uncredited) |
 | Ross Bagdasarian, Jr. als Alvin (singing voice) (uncredited) |
 | Janice Karman als Theodore / The Chipettes (singing voice) (uncredited) |
 | Steve Vining als Simon (singing voice) (uncredited) |
 | Courtney Galiano als Eleanor - Performer (voice) (uncredited) |
 | Gee Alexander als Simon Dance Double (uncredited) |
| Mark Alkofer als Football Referee (uncredited) |
 | Tallie Brinson als Principal Dancer (uncredited) |
| Ericka Clevenger als Cheerleader (uncredited) |
| Sean Decker als Dodgeball Player (uncredited) |
| Sarah Fontenot als Cheerleader (uncredited) |
| Paul Gonzales als Charice Bassist (uncredited) |
| David Lautman als High School Student (uncredited) |
 | Casper Smart als Dancer (uncredited) |
| Andrew Thompson als Stadium Announcer (uncredited) |
| Doug Jackson | Sound Effects Editor |
| Arnon Milchan | Executive Producer |
| David Newman | Original Music Composer |
| Karen Rosenfelt | Executive Producer |
| Jeff Carson | Music Editor |
| Juel Bestrop | Casting |
| Anthony B. Richmond | Director of Photography |
| Jon Vitti | Writer |
| Cheri Minns | Makeup Department Head |
| Betty Thomas | Director |
| Ross Bagdasarian, Jr. | Producer |
| Janice Karman | Producer |
| Ross Bagdasarian | Characters |
| Michele Imperato | Executive Producer |
| Michele Imperato | Unit Production Manager |
| Steve Waterman | Executive Producer |
| Julianne Jordan | Music Supervisor |
| Alexandra Welker | Costume Design |
| R.J. Kizer | ADR Editor |
| Matthew Friedman | Editor |
| David Betancourt | Foley Mixer |
| Scott Curtis | Foley Editor |
| Dawn Lunsford | Foley Artist |
| Tim Gomillion | Sound Recordist |
| Galen Goodpaster | First Assistant Sound Editor |
| Mildred Iatrou | Supervising Sound Editor |
| David Lucarelli | Sound Recordist |
| Chuck Michael | Sound Effects Editor |
| John Murray | Foley Supervisor |
| Alicia Stevenson | Foley Artist |
| Glenn Berger | Writer |
| Jonathan Aibel | Writer |
| Seth Yanklewitz | Casting |
| Paul Sonski | Set Designer |
| Steven Lambert | Stunts |
| Michael Keller | Sound Re-Recording Mixer |
| Raman Rao | Rigging Gaffer |
| Nancy MacLeod | Sound Effects Editor |
| Steven R. Mathis | Gaffer |
| David 'Goldy' Goldsmith | Video Assist Operator |
| Marcia Hinds | Production Design |
| Bo Johnson | Art Direction |
| Dan DiPrima | Supervising Music Editor |
| David Lombard | Leadman |
| Jaimie Trueblood | Still Photographer |
| Gary C. Bourgeois | Sound Re-Recording Mixer |
| Lori McCoy-Bell | Hair Department Head |
| Laura Graham | ADR Editor |
| Merribelle Anderson | Key Hair Stylist |
| Michael J. Benavente | Supervising Sound Editor |
| Rebecca Robertson | Script Supervisor |
| Gary A. Theard | Boom Operator |
| Alan E. Lorimer | Special Effects Supervisor |
| Leigh Shanta | Unit Production Manager |
| Guy Bar'ely | Animation |
| Tim Lauber | Sound Recordist |
| Christine Sirois | Sound Recordist |
| Julie D'Antoni | Visual Effects |
| John Moio | Stunt Coordinator |
| Willie D. Burton | Sound Mixer |
| Glenn Brown | Steadicam Operator |
| Karen Agresti | Set Decoration |
| Michael Axinn | ADR Editor |
| Elena Arroy | Key Makeup Artist |
| Jules Carideo | Production Coordinator |
| Rosero McCoy | Choreographer |
| Jesse Johnstone | Sound Recordist |
| Julie-Marie Robar | Costume Supervisor |
| Maggie Elliott | Makeup Artist |
| Justin Elterman | Graphic Designer |
| Michael Doherty | Stand In |
| Lars Petersen | Construction Coordinator |
| Ron Tondreau | Key Grip |
