 | Miyuki Sawashiro als Natasha Romanoff / Black Widow (voice) |
 | Tesshou Genda als Frank Castle / The Punisher (voice) |
 | Hideaki Tezuka als Nick Fury (voice) |
 | Hiroki Touchi als Elihas Starr (voice) |
 | Ryuuzaburou Ootomo als Cain (voice) |
 | Junko Minagawa als Maria Hill (voice) |
 | Daisuke Namikawa als Amadeus Cho (voice) |
 | Masashi Sugawara als Orion (voice) |
 | Yuichi Karasuma als The Hulk (voice) |
| Hisashi Izumi als Ren (voice) |
 | Keiji Fujiwara als Tony Stark / Iron Man (voice) |
 | Jennifer Carpenter als Natasha Romanoff / Black Widow (voice) |
 | Brian Bloom als Frank Castle / Punisher (voice) |
 | John Eric Bentley als Nick Fury (voice) |
 | Grant George als Elihas Starr (voice) |
 | Kyle Hebert als Cain (voice) |
 | Kari Wahlgren als Maria Hill (voice) |
 | Eric Bauza als Amadeus Cho (voice) |
 | J. B. Blanc als Orion (voice) |
 | Fred Tatasciore als The Hulk / Ren (voice) |
 | Matthew Mercer als Tony Stark / Iron Man (voice) |
| Stan Lee | Characters |
| Stan Lee | Co-Executive Producer |
| Masafumi Mima | Sound Director |
| Masao Takiyama | Executive Producer |
| Alan Fine | Executive Producer |
| Jeph Loeb | Executive Producer |
| Toshihiko Nakajima | Sound Director |
| Dan Buckley | Executive Producer |
| Tetsuya Takahashi | Original Music Composer |
| Joe Quesada | Co-Executive Producer |
| Mitsutaka Hirota | Screenplay |
| Kenichi Shimizu | Director |
| Kenichi Shimizu | Storyboard Artist |
| Marjorie Liu | Story |
| Kazuyuki Hashimoto | Art Direction |
| Kashiko Kimura | Editor |
| Kenji Fujita | Director of Photography |
| Simon Philips | Executive Producer |
| Shuhei Yabuta | 3D Director |
| Yukie Noguchi | Color Designer |
| Hiroyuki Okada | Executive Producer |
| Taro Morishima | Producer |
| Megan Thomas Bradner | Producer |
| Harrison Wilcox | Producer |
| Mariko Tsukatsune | Editor |
| Scott Dolph | Producer |
| Hitoshi Haga | Storyboard Artist |
| Makoto Fuchigami | Storyboard Artist |
| You Moriyama | Conceptual Design |
| Shin Inoie | Special Effects |
| Cindy H. Yamauchi | Animation Director |
| Akemi Kobayashi | Animation Director |
| Kenichi Shima | Animation Director |
