 | Kevin Conroy als Batman / Bruce Wayne (voice) |
 | Melissa Rauch als Harley Quinn (voice) |
 | Paget Brewster als Pamela Isley / Poison Ivy (voice) |
 | Loren Lester als Nightwing / Dick Grayson (voice) |
 | Kevin Michael Richardson als Floronic Man / Jason Woodrue (voice) |
 | Eric Bauza als Wesley (voice) |
 | Trevor Devall als Bobby Liebowitz (voice) |
 | John DiMaggio als Dr. Alec Holland / Swamp Thing (voice) / Sarge Steel (voice) |
 | Robin Atkin Downes als Rhino (voice) |
 | Rob Paulsen als Harold Goldblum (voice) |
 | Mindy Sterling als Project Supervisor (voice) |
 | Bruce Timm als Booster Gold (voice) |
| Paul Dini | Characters |
| Alan Burnett | Co-Producer |
| Christopher D. Lozinski | Editor |
| Sam Register | Executive Producer |
| Michael Uslan | Executive Producer |
| Bruce Timm | Story |
| Wes Gleason | Casting |
| Bruce Timm | Executive Producer |
| Benjamin Melniker | Executive Producer |
| Sam Liu | Director |
| Michael McCuistion | Original Music Composer |
| Kristopher Carter | Original Music Composer |
| Lolita Ritmanis | Original Music Composer |
| Amy McKenna | Line Producer |
| James Krieg | Teleplay |
| Bruce Timm | Teleplay |
| Matthew Mahoney | Associate Producer |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| David M. Cowan | Dialogue Editor |
| David M. Cowan | ADR Editor |
| Kelly Ann Foley | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Michael Garcia | ADR Editor |
| Darren Griffiths | Online Editor |
| Michael Garcia | Dialogue Editor |
| Liz Carroll | Administration |
| Bobbie Page | Production Supervisor |
| Ed Adams | Production Manager |
| Bob Kane | Characters |
| Bill Finger | Characters |
| Dan Jurgens | Characters |
| Bruce Timm | Characters |
| Marv Wolfman | Characters |
| George Pérez | Characters |
| Len Wein | Characters |
| Bernie Wrightson | Characters |
