 | Jensen Ackles als Bruce Wayne / Batman (voice) |
 | Josh Duhamel als Harvey Dent / Two-Face (voice) |
 | Naya Rivera als Selina Kyle / Catwoman (voice) |
 | Billy Burke als Commissioner James Gordon (voice) |
 | Titus Welliver als Carmine 'The Roman' Falcone / Luigi Maroni (voice) |
 | David Dastmalchian als Julian Day / Calendar Man / Oswald Cobblepot / Penguin (voice) |
 | Julie Nathanson als Gilda Dent (voice) |
 | Alastair Duncan als Alfred Pennyworth (voice) |
 | Katee Sackhoff als Pamela Isley / Poison Ivy (voice) |
 | Robin Atkin Downes als Jonathan Crane / Scarecrow / Thomas Wayne (voice) |
 | John DiMaggio als Jervis Tetch / Mad Hatter (voice) |
 | Laila Berzins als Sofia Falcone (voice) |
 | Fred Tatasciore als Solomon Grundy / Vincent Falcone (voice) |
 | Amy Landecker als Barbara Gordon / Carla Viti (voice) |
 | Troy Baker als Joker (voice) |
 | Alyssa Diaz als Renee Montoya (voice) |
 | Gary LeRoi Gray als Pearce (voice) |
 | Jim Pirri als Sal Maroni (voice) |
 | Zach Callison als Young Bruce Wayne (voice) |
 | Rick D. Wasserman als Bodyguard (voice) |
| Frank Miller | Characters |
| Bob Kane | Characters |
| Michael Uslan | Executive Producer |
| Jeph Loeb | Graphic Novel |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Butch Lukic | Supervising Producer |
| Bobbie Page | Production Supervisor |
| Sam Register | Executive Producer |
| James Krieg | Producer |
| Bill Finger | Characters |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Darren Griffiths | Online Editor |
| Michael Gatt | Original Music Composer |
| Wes Gleason | Casting |
| Tim Sheridan | Writer |
| Sol Choi | Storyboard Artist |
| Dan Duncan | Storyboard Artist |
| Sarah E. Noonan | Casting |
| Kimberly S. Moreau | Producer |
| Elsa Garagarza | Storyboard Artist |
| Peter Girardi | Executive In Charge Of Production |
| Brian E.S. Jones | Executive In Charge Of Production |
| Augusto Barranco | Background Designer |
| Owen Sullivan | Storyboard Artist |
| Bruce King | Editor |
| Matthew Girardi | Art Direction |
| Chris Palmer | Director |
| Bob Smith | Storyboard Artist |
| Matthew Bordenave | Storyboard Artist |
| Sung Shin | Storyboard Artist |
| Julie Osborn | Post Producer |
| David Mazzucchelli | Characters |
| Elliot Byrne | Background Designer |
| Marlene Corpuz | Production Manager |
| Sharon Yvonne Lopez | Visual Effects Production Manager |
| Mark A. Keatts | Supervising ADR Editor |
| Mark A. Keatts | Supervising Dialogue Editor |
| Ed Adams | Production Manager |
| Tim Sale | Graphic Novel |
| Tim Ridley | Background Designer |
| Tec Manalac | Background Designer |
| Agnes Kim | Casting |
| Adrian Barrios | Storyboard Artist |
| Alicia Heraper | Assistant Production Manager |
| Dou Hong | Character Designer |
| Otto Schmidt | Character Designer |
| Art Lee | Prop Designer |
| Brian Uchida | Prop Designer |
| Assaf Meir | Background Designer |
| John Paik | Background Designer |
| Vince Toyama | Background Designer |
| Kim Bowen | Color Designer |
| Victoria Thornberry | Color Designer |
| David Maximo | Storyboard Artist |
| Jose Andres Velasco | Storyboard Artist |
| Rozlynn Waltz | Storyboard Artist |
| Anthony Akira Andrade | Animation |
| Brett Hardin | Animation |
| Aric Mannion | Animation |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| Angela O'Sullivan | Line Producer |
| Dusty Abell | Character Designer |
| Tina Duong | Character Designer |
| Pakin Liptawat | Background Designer |
| Craig Cuqro | Color Designer |
| Gabe Lee | Storyboard Artist |
| Brenna Kraus | Title Designer |
| Brenna Kraus | Graphic Designer |
| Kip Brown | Post Producer |
| Harrison T. Barth | Assistant Editor |
| Michele Beeson | Assistant Editor |
| Gabriel Gianola | Assistant Editor |
