 | Wesley Snipes als Eric Brooks / Blade |
 | Jessica Biel als Abigail Whistler |
 | Ryan Reynolds als Hannibal King |
 | Kris Kristofferson als Abraham Whistler |
 | Dominic Purcell als Dracula / Drake |
 | Parker Posey als Danica Talos |
 | Natasha Lyonne als Sommerfield |
 | James Remar als Ray Cumberland |
 | John Michael Higgins als Dr. Edgar Vance |
 | Patton Oswalt als Hedges |
 | Callum Keith Rennie als Asher Talos |
 | Paul Michael Lévesque als Jarko Grimwood |
 | Françoise Yip als Virago |
 | Mark Berry als Chief Martin Vreede |
 | Paul Anthony als Wolfe |
 | Michael Anthony Rawlins als Wilson Hale |
 | Ginger Page als Zoe |
 | Eric Bogosian als Bentley Tittle |
 | Erica Cerra als Goth Vixen Wannabe |
 | Clay Cullen als Stone |
| Shannon Powell als Woman Bystander |
 | Birkett Turton als Dingo |
 | Brian Steele als Drake Creature (uncredited) |
 | Christopher Heyerdahl als Caulder |
| David S. Goyer | Director |
| David S. Goyer | Writer |
| Marv Wolfman | Characters |
| Gene Colan | Characters |
| Peter Frankfurt | Producer |
| David S. Goyer | Producer |
| Lynn Harris | Producer |
| Avi Arad | Executive Producer |
| Cale Boyter | Executive Producer |
| Toby Emmerich | Executive Producer |
| Stan Lee | Executive Producer |
| Ramin Djawadi | Original Music Composer |
| RZA | Original Music Composer |
| Gabriel Beristain | Director of Photography |
| Conrad Smart | Editor |
| Howard E. Smith | Editor |
| Ronnie Yeskel | Casting |
| Laura Jean Shannon | Costume Design |
| Art Schaefer | Co-Producer |
| Wesley Snipes | Producer |
| Lucrezia Casta | Production Design |
| Chris Gorak | Production Design |
| Patrick Banister | Art Direction |
| Eric Fraser | Art Direction |
| Jamie Jonasson | Set Decoration |
| Tedd Kuchera | Set Decoration |
| Lisa Love | Makeup Artist |
| Sydney Silvert | Makeup Artist |
| Danna Rutherford | Hairstylist |
| George A. Grieve | Production Manager |
| Sara Romilly | Post Production Supervisor |
| Yoichi Art Sakamoto | Makeup Effects |
| Harlow MacFarlane | Makeup Effects |
| Lin-Li-Anne Lee | Art Department Coordinator |
| Nancy Ford | Assistant Art Director |
| Chris Claridge | Construction Coordinator |
| Andrei Andrianko | Set Designer |
| Eileen Cunningham | Scenic Artist |
| Sheila Millar | Set Designer |
| Perry Battista | Leadman |
| Vern Lavoie | Lead Painter |
| Joe Wilmott | Lead Painter |
| Jordu Schell | Sculptor |
| Max Chickite | Sculptor |
| Aaron Glascock | Supervising Sound Editor |
| Curt Schulkey | Supervising Sound Editor |
| Stephen Hunter Flick | Sound Effects Editor |
| Ezra Dweck | Sound Effects Editor |
| Piero Mura | Sound Effects Editor |
| John Joseph Thomas | Sound Effects Editor |
| Marvin Walowitz | Sound Effects Editor |
| Aaron Glascock | Sound Re-Recording Mixer |
| Skip Lievsay | Sound Re-Recording Mixer |
| Rory Cutler | Special Effects Coordinator |
| Alexandra Altrocchi | Visual Effects Producer |
| Joseph B. Conmy IV | Visual Effects Producer |
| Chris Del Conte | Visual Effects Producer |
| Vicki Galloway Weimer | Visual Effects Producer |
| Meredith Meyer-Nichols | Visual Effects Producer |
| Desi Ortiz | Visual Effects Editor |
| Paul M. Wagner | Visual Effects Editor |
| John Vegher | Visual Effects Supervisor |
| Joe Bauer | Visual Effects Supervisor |
| Ryan Ennis | Stunt Coordinator |
| Larry Lam | Stunt Coordinator |
| Mark Chow | Camera Operator |
| Marco Ciccone | Camera Operator |
| Lou Gruzelier | Camera Operator |
| Richard Wilson | Camera Operator |
| Diyah Pera | Still Photographer |
| Craig Fikse | Steadicam Operator |
| Jeff Upton | Gaffer |
| Bill Kassis | Gaffer |
| Lance Fisher | Additional Camera |
| Luisa Dalmagro | Set Costumer |
| Susan O'Hara | Set Costumer |
| Lee Sollenberger | Set Costumer |
| Faye Sevilla | Costume Supervisor |
| Karin Graziani | Digital Intermediate |
| Brad Reinke | Digital Intermediate |
| Alex Gibson | Music Editor |
| Tim Abbatoye | Transportation Coordinator |
| Mike Sassen | Transportation Coordinator |
| Michael C. Young | Location Manager |
| Julia Frittaion | Unit Publicist |
| Tracey Shelley-Lavery | Studio Teachers |
| Steven Bernstein | Additional Photography |
| James Bamford | Stunts |
| Eddie Perez | Stunt Coordinator |
| Anji Bemben | Key Hair Stylist |
| Paul Herndon | Conceptual Design |
| Doug Parton | Construction Foreman |
| Lenore Nemani | Location Manager |
| Raymond Alley | Painter |
| Martin Emond | Production Illustrator |
| David Pirrie | Standby Painter |
| Steven J. Winslow | Camera Technician |
| Alan Cohen | First Assistant Camera |
| James L. Hurford | Key Grip |
| Grant Hamakawa | Grip |
| Nancy Duggan | Assistant Costume Designer |
| Miya Dube | Set Dressing Artist |
| Ryan Steacy | Armorer |
| John Beatty | Carpenter |
| Barb Foster | Chef |
| Lisa Ahlers | Craft Service |
| Everett Burrell | Digital Effects Supervisor |
| Paul Amenta | Driver |
| Paul Prokop | Executive in Charge of Finance |
| Bart Fisher | Post Production Assistant |
| Jonathan Davidson | Production Controller |
| John Lavell | Propmaker |
| Robert Milicevic | Security |
| Lennox Brown | Stand In |
| Joe Boswell | Systems Administrators & Support |
| Deb Asch | Technical Supervisor |
| Tim Fortin | Transportation Captain |
| Harley Ware | Transportation Co-Captain |
| Art Schaefer | Unit Production Manager |
| Colin Decker | Utility Stunts |
| Pete Whyte | First Assistant Director |
| Jim Passon | Color Timer |
| Mindy Elliott | First Assistant Editor |
| Chris Cochrane | Best Boy Electric |
| David Ghegan | Electrician |
| Rod Newby | Rigging Gaffer |
| Herb DeWaal | Rigging Grip |
| Caroline Liem | Casting Associate |
| Erik Holmberg | Executive In Charge Of Production |
| Karen Eisenstadt | Production Accountant |
| Troy James Miller | Production Coordinator |
| Brian Walsh | Production Supervisor |
| Dave Griffiths | Boom Operator |
| Oscar Mitt | Assistant Sound Editor |
| George Drakoulias | Music Supervisor |
| Lindsay Bucknell | Sound Mixer |
| Gregory Arbit | 3D Artist |
| Akira Orikasa | Animation Supervisor |
| Rob Meyers | Digital Compositors |
| Jeff Varga | Visual Effects |
| Mark Edwards | Visual Effects Coordinator |
| Jason Crosby | CG Supervisor |
| Kira Roessler | Dialogue Editor |
| Andy Nieman | Property Master |
| Steve Koster | Aerial Director of Photography |
| John Roesch | Foley Artist |
| Alyson Dee Moore | Foley Artist |
| Rick Canelli | ADR Recordist |
| Mary Jo Lang | Foley Mixer |
| Thomas J. O'Connell | ADR Mixer |
| Mark Hensley | ADR Mixer |
| Greg Steele | ADR Mixer |
| Patrick Kalyn | Pre-Visualization Supervisor |
| Rebecca Lee | Assistant Makeup Artist |
| Rob Miller | Contact Lens Technician |
| Dawn Climie | Costume Set Supervisor |
| Andre Brouwer | Truck Costumer |
| Vic Armstrong | Action Director |
| Cindy Smith | Second Assistant Director |
| Eddie Perez | Second Unit Director |
| Ryan Carpenter | Third Assistant Director |
| Donna Smith | Assistant Property Master |
| TyRuben Ellingson | Concept Artist |
| Jonathan Jakubec | Construction Buyer |
| Leah Hong | Graphic Designer |
| Dinesh Gill | Property Buyer |
| Darrin Denlinger | Storyboard Artist |
| Scott Morgan | Foley Recordist |
| John Joseph Thomas | Foley Supervisor |
| Naan Spiess | Sound Assistant |
| Steve Fox | Special Effects Assistant |
| John Reynolds | Special Effects Best Boy |
| Adam Tayler | Special Effects Technician |
| Matt Russell | Visual Effects Production Assistant |
| Greg Baxter | Visual Effects Production Manager |
| Trevor Jones | Stunt Double |
| John Ashker | Stunt Driver |
| Dennis Skog | Best Boy Grip |
| Vanessa Andrascik | Camera Loader |
| Martin Udo Haas | Dolly Grip |
| Heidi Buecking | Second Assistant Camera |
| Danny Braet | Lead Animator |
| Jeremy Oddo | Senior Animator |
| Errin Lally | Casting Assistant |
| Alison Fraser | Wardrobe Supervisor |
| Seth Clark | Assistant Editor |
| Jill Bogdanowicz | Digital Intermediate Colorist |
| Linda Williams | Digital Intermediate Editor |
| Gary Burritt | Negative Cutter |
| Betty Burkhart | Post Production Coordinator |
| Sarah Done | Assistant Location Manager |
| Ginger Joyce | Accountant |
| Mark Dumas | Animal Coordinator |
| Sean Goojha | Art Department Production Assistant |
| Rob Gibbs | Assistant Chef |
| Denise Love | Assistant Production Coordinator |
| Niles Heckman | Compositing Supervisor |
| Rob Meyers | Digital Supervisor |
| Chuck Jeffreys | Fight Choreographer |
| Sylvia Jang | First Assistant Accountant |
| Jason Fischer | First Assistant Production Coordinator |
| Ray Scalice | General Manager |
| John Dines | Generator Operator |
| Derry Frost | Head of Production |
| Adrian Diepold | Key Set Production Assistant |
| Deak Ferrand | Matte Painter |
| Jeff Miller | Production Assistant |
| Leon Dudevoir | Production Executive |
| Anthony Alvaro | Production Secretary |
| Howard Blank | Technical Advisor |
| Yuka Kobayashi | Stunts |
| Miya Dube | Set Dresser |
| Paul Goodwin | Set Dresser |
| Brant Lindroos | Set Dresser |
| Mike Marsten | Set Dresser |
| Colin Meacham | Set Dresser |
| Guy Miller | Set Dresser |
| Ocea Ringrose | Set Dresser |
| Tracy B. Dunlop | Set Dresser |
| Matthew Clancy | Standby Carpenter |
| James Doh | Storyboard Artist |
| Natasha Young | Script Supervisor |
| Amanda Alexander | Script Supervisor |
| Jeannine Dupuy | Script Supervisor |
| Gary Archer | Prosthetics |
| Doug Chapman | Stunts |
| Corry Glass | Stunts |
| Ruby J. Munro | Script Supervisor |
| Andrea Kinsky | Stunts |
| Brett Armstrong | Stunts |
| Krista Bell | Stunts |
| Steven McMichael | Stunt Double |
| Joanne Leach | Stunts |
| Clint Carleton | Stunts |
| Don Lew | Stunts |
| Jon Kralt | Stunts |
| Gerald Paetz | Stunts |
| Ryan Ennis | Stunts |
| Glenn Ennis | Stunts |
| Caroline Field | Stunts |
| Brian Ho | Stunts |
| Tom Glass | Stunts |
| Brad Kelly | Stunts |
| Clay Donahue Fontenot | Stunt Coordinator |
| Kimberly Chiang | Stunts |
| Alex Chiang | Stunts |
| Garvin Cross | Stunts |
| Mark Aisbett | Stunts |
| Lloyd Adams | Stunts |
| Scott J. Ateah | Stunts |
| Aaron Au | Stunts |
| Curt Bonn | Stunts |
| Loyd Bateman | Stunts |
| Leanne Buchanan | Stunt Double |
| Rob 'Sluggo' Boyce | Stunts |
| Mike Carpenter | Stunts |
| Clint Cadinha | Fight Choreographer |
| Mike Cook | Stunts |
| Joe Doserro | Stunts |
| Larry Lam | Stunts |
| Darren McGuire | Stunts |
| Trevor Jones | Stunt Double |
| Todd Scott | Stunts |
| Darryl Scheelar | Stunts |
| Hugo Steele | Stunts |
| Rob Wilton | Stunts |
| Colby Chartrand | Stunts |
| Mike Desabrais | Stunts |
| Gloria O'Brien | Stunt Double |
| Shawn Orr | Stunts |
| Sharon Simms | Stunts |
| Sylvesta Stuart | Stunts |
| Jeff Sanca | Stunts |
| Rorelee Tio | Stunts |
| Ryan Schroeder | Stunts |
| Claire Webb | Stunts |
| Terrance Leigh | Stunts |
| Scott Nicholson | Stunts |
| Christopher Gordon | Stunts |
| Mark Chin | Stunts |
| Mike Ching | Stunts |
| Dean Choe | Stunts |
| Janina Dall | Stunts |
