 | Bud Abbott als Chick Young |
 | Lou Costello als Wilbur Grey |
 | Lon Chaney Jr. als Larry Talbot/The Wolf Man |
 | Bela Lugosi als Count Dracula |
 | Glenn Strange als The Frankenstein Monster |
 | Lenore Aubert als Dr. Sandra Mornay |
 | Jane Randolph als Joan Raymond |
 | Frank Ferguson als Mr. McDougal |
 | Charles Bradstreet als Dr. Stevens |
 | George Barton als Man |
 | Vincent Price als The Invisible Man (voice) |
 | Bobby Barber als Waiter |
 | Joe Kirk als Man at Costume Party in Fez |
| Harry Brown als Photographer |
 | Howard Negley als Harris - Insurance Man |
 | Carl Sklover als Man at Costume Party |
 | Helen Spring als Woman at Baggage Counter |
| Paul Stader als Sergeant |
| Clarence Straight als Man in Armor |
| Joe Walls als Man at Costume Party |
| Charles Barton | Director |
| Robert Lees | Screenplay |
| Frederic I. Rinaldo | Screenplay |
| John Grant | Screenplay |
| Robert Arthur | Producer |
| Charles Van Enger | Director of Photography |
| Frank Gross | Editor |
| Oliver Emert | Set Decoration |
| Russell A. Gausman | Set Decoration |
| Grace Houston | Costume Design |
| Bud Westmore | Makeup Artist |
| Jack Kevan | Makeup Artist |
| Carmen Dirigo | Hairstylist |
| Mary Shelley | Characters |
| Bram Stoker | Characters |
| Emile LaVigne | Makeup Artist |
| Frank Skinner | Original Music Composer |
| Robert Pierce | Camera Operator |
| Leslie I. Carey | Sound |
| Robert Pritchard | Sound |
| Walter Lantz | Animation Director |
| Hilyard M. Brown | Art Direction |
| Bernard Herzbrun | Art Direction |
| Joseph E. Kenney | Assistant Director |
| Henry Spitz | Production Manager |
| Jerome Ash | Special Effects |
| David S. Horsley | Special Effects |
| Fred Knoth | Special Effects |
| Eddie Parker | Stunts |
| Helen Thurston | Stunts |
| Glenn Adams | Still Photographer |
| Laverne Harding | Animation |
| Les Kline | Animation |
| Ed Love | Animation |
| Dick Lundy | Animation Director |
| David Tamkin | Orchestrator |
| Betty A. Griffin | Script Supervisor |
| Norman Abbott | Dialogue Coach |
| Walter Lantz | Title Designer |
