 | Paul Bettany als Charles Darwin |
 | Jennifer Connelly als Emma Darwin |
 | Martha West als Anne Darwin |
 | Guy Henry als Technician |
 | Jeremy Northam als John Brodie-Innes |
 | Toby Jones als Thomas Henry Huxley |
 | Benedict Cumberbatch als Joseph Dalton Hooker |
 | Jim Carter als Parslow |
 | Robert Glenister als Dr. Holland |
 | Ian Mercer als Goodman |
 | Bill Paterson als Dr. Gully |
 | Teresa Churcher als Mrs. Davies |
 | Richard Ridings als Thatcher |
 | Freya Parks als Etty Darwin |
 | Ian Kelly als Captain Fitzroy |
| Anabolena Rodriguez als Fuegia Basket |
| Paul Campbell als Boat Memory |
| Zak Davies als Jemmy Button |
| Christopher Dunkin als George Darwin |
| Gene Goodman als Franky Darwin |
| Harrison Sansostri als Lenny Darwin |
| Jon Amiel | Director |
| David M. Thompson | Executive Producer |
| Christina Yao | Executive Producer |
| Jess Hall | Director of Photography |
| Nick O'Hagan | Producer |
| Jamie Laurenson | Executive Producer |
| Melanie Oliver | Editor |
| Pip Ayers | Producer |
| Randal Keynes | Novel |
| Jon Amiel | Screenstory |
| Laurence Dorman | Production Design |
| Christopher Young | Original Music Composer |
| John Collee | Screenplay |
| Jeremy Thomas | Producer |
| Janice Eymann | Executive Producer |
| Peter Watson | Executive Producer |
| Tom Waller | Line Producer |
| John Collee | Screenstory |
| Celestia Fox | Casting |
| Sara Desmond | Unit Production Manager |
| Louise Stjernsward | Costume Design |
| Gary Jopling | Art Direction |
| Bill Crutcher | Supervising Art Director |
| Dominic Capon | Set Decoration |
| Francesco Alberico | Hairstylist |
| Vitchu Chavasit | Makeup Artist |
| Ashley Johnson | Makeup Artist |
| Veronica McAleer | Makeup Artist |
| Peter Gleaves | ADR Mixer |
| Sue Harding | Foley Artist |
| Paul Hanks | Foley Artist |
| Jack Stew | Foley Artist |
| Ruth Sullivan | Foley Artist |
| Chris Burdon | Sound Re-Recording Mixer |
