 | Ed Asner als J. Jonah Jameson (voice) |
 | Dorian Harewood als Tombstone (voice) |
 | Christopher Daniel Barnes als Spider-Man/Peter Parker (voice) |
 | Sara Ballantine als Mary Jane Watson (voice) |
 | Leigh-Allyn Baker als Alisha Silvermain (voice) |
 | Maxwell Caulfield als Alistair Smythe (voice) |
 | Linda Gary als Tante May Parker (voice) |
 | Gary Imhoff als Harry Osborn (voice) |
 | Roscoe Lee Browne als Kingpin/Wilson Fisk (voice) |
| Steve Ditko | Characters |
| Shuki Levy | Original Music Composer |
| Bob Richardson | Supervising Producer |
| Udi Harpaz | Original Music Composer |
| Cynthia Harrison | Writer |
| Haim Saban | Original Music Composer |
| Bob Richardson | Director |
| Mark Hoffmeier | Story |
| Stan Lee | Executive Producer |
| Stan Lee | Characters |
| Brooks Wachtel | Writer |
| Sean Catherine Derek | Writer |
