 | Antonio Banderas als Puss in Boots (voice) |
 | Salma Hayek als Kitty Softpaws (voice) |
 | Harvey Guillén als Perrito (voice) |
 | Wagner Moura als Wolf (voice) |
 | Florence Pugh als Goldilocks (voice) |
 | Olivia Colman als Mama Bear (voice) |
 | Ray Winstone als Papa Bear (voice) |
 | Samson Kayo als Baby Bear (voice) |
 | John Mulaney als Jack Horner (voice) |
 | Da'Vine Joy Randolph als Mama Luna (voice) |
 | Anthony Mendez als Doctor (voice) |
 | Kevin McCann als Ethical Bug (voice) |
 | Bernardo de Paula als Governor / Little Boy's Father / Male Partier (voice) |
 | Betsy Sodaro als Jo Serpent / Additional Baker's Dozen Gang Member (voice) |
 | Artemis Pebdani als Jan Serpent (voice) |
 | Conrad Vernon als Gingy (voice) |
 | Cody Cameron als Pinocchio (voice) |
 | Kailey Crawford als Little Goldi (voice) |
 | Al Rodrigo als Bartender (voice) |
 | Bob Persichetti als Ohhh Cat (voice) |
| Miguel Matrai als Little Boy (voice) |
 | Pilar Uribe als Governor's Assistant / Female Partier (voice) |
 | Heidi Gardner als Last Baker (voice) |
 | Joel Crawford als Band Member / Baker's Dozen Gang Member (voice) |
| Januel Mercado als Band Member / Baker's Dozen Gang Member (voice) |
| James Ryan als Little Jack Audience Member / Baker's Dozen Gang Member (voice) |
| Natalia Cronembold als Waitress / Baker's Dozen Gang Member (voice) |
| Paul Fisher als Additional Baker's Dozen Gang Member (voice) |
 | Aydrea Walden als Additional Baker's Dozen Gang Member (voice) |
| Sheri Galloway | Assistant Editor |
| Andrew Adamson | Executive Producer |
| Christopher Meledandri | Executive Producer |
| Cody Cameron | Storyboard Artist |
| Tommy Swerdlow | Screenplay |
| Tommy Swerdlow | Story |
| Greg P. Russell | Sound Re-Recording Mixer |
| Simon Wells | Storyboard Artist |
| R. Orlando Duenas | Associate Editor |
| Etan Cohen | Co-Writer |
| Heitor Pereira | Original Music Composer |
| Jeff Biancalana | Storyboard Artist |
| Bill Riling | Storyboard Artist |
| Chris Mitchell | Storyboard Artist |
| John Puglisi | Storyboard Artist |
| Julian Slater | Supervising Sound Editor |
| Julian Slater | Sound Re-Recording Mixer |
| Paul Fisher | Screenplay |
| Tom Wheeler | Story |
| Karl Armstrong | Associate Editor |
| James Ryan | Editor |
| David Lisbe | Animation |
| Chris Stover | Head of Layout |
| Julien Bocabeille | Animation Supervisor |
| Garrett O'Neal | Animation |
| Tim Walston | Sound Designer |
| Paul Pirola | Foley Supervisor |
| Christi Soper Hilt | Casting |
| Gaby Moreno | Songs |
| Rick Hromadka | Sound Designer |
| Jason W. Jennings | Sound Designer |
| Jason W. Jennings | Supervising Sound Editor |
| Dan Navarro | Songs |
| Anthony Zierhut | Storyboard Artist |
| Linda Yeaney | First Assistant Sound Editor |
| Joel Crawford | Director |
| Matt Flynn | Storyboard Artist |
| Colin Jack | Storyboard Artist |
| Brian Menz | Animation |
| Warren Leonhardt | Storyboard Artist |
| Jeff Williams | Animation |
| Shiyoon Kim | Character Designer |
| John Wong | Animation |
| Marek Kochout | Animation |
| Laurent Caneiro | Animation Supervisor |
| Bill Diaz | Animation |
| Willy Harber | Animation Supervisor |
| Rani Naamani | Animation |
| Mark Edwards | Visual Effects Supervisor |
| Ashley Lambert | ADR Voice Casting |
| Andrea Blasich | Character Designer |
| Alexander Snow | Animation |
| Gavin Greenaway | Conductor |
| Dane Stogner | Animation Supervisor |
| Claire Keane | Visual Development |
| Ludovic Bouancheau | Head of Animation |
| Ken McGill | Sound Effects Editor |
| Fabio Lignini | Animation Supervisor |
| Dan Wagner | Animation Supervisor |
| Chadd Ferron | Animation |
| Heidi Jo Gilbert | Head of Story |
| Evon Freeman | Storyboard Artist |
| Johane Matte | Storyboard Artist |
| Neil Campbell Ross | Visual Development |
| KAROL G | Songs |
| Ryan Squires | Foley Recordist |
| Robert Christian Huth | Animation |
| Patrick Danaher | Animation |
| Daniel Edwards | Animation |
| Jorge García | Lead Animator |
| Ryan Page | Animation |
| William Salazar | Animation |
| Steven Hornby | Animation |
| Philippe Le Brun | Animation |
| Antony Gray | Lead Animator |
| Martin P. Hopkins | Animation |
| Tyler Phillips | Animation |
| Marco Regina | Animation |
| JP Sans | Animation |
| Ares Deveaux | Animation |
| Andrew Harkins | Animation |
| Nate Wragg | Production Design |
| Adrien Liv | Animation |
| Yung Pham | Animation |
| David Couchariere | Animation |
| Sean Sexton | Animation |
| Greg Whittaker | Animation |
| Michael Lynn | Production Manager |
| Jakob Hjort Jensen | Animation Supervisor |
| Thomas Grummt | Lead Animator |
| Hans Dastrup | Animation |
| John Geller | Post Production Supervisor |
| Michelle Cowart | Animation |
| David Polk | Animation |
| Sebastien Wojda | Animation |
| David Wolter | Storyboard Artist |
| Robyne Powell | Animation |
| Justin Weg | Animation |
| Reece Porter | Animation |
| Ivan Oviedo | Animation |
| Mark Swift | Producer |
| Natalia Cronembold | Associate Editor |
| Mark Roennigke | Animation |
| Drew Adams | Animation |
| Patrick Giusiano | Animation |
| Jacquelyn Karambelas | Associate Editor |
| Heather Lanza | Associate Producer |
| Onur Yeldan | Animation |
| Michael Losure | Effects Supervisor |
| David Bleich | Visual Development |
| Elisabeth Franklin | Animation |
| Joseph Chong | Animation |
| Ryan Vicik | Animation |
| Steve Vanseth | Animation |
| Taylor Meacham | Storyboard Artist |
| Prashanth Cavale | Animation |
| Hanna Kim | Storyboard Artist |
| Steven R.V. Johnson | Animation |
| Aaron Austin | Storyboard Artist |
| David Shipps | Orchestrator |
| Jeremy Schaefer | Animation |
| Steven A. MacLeod | Storyboard Artist |
| Sam Rogers | Foley Artist |
| Shir Baron | Animation |
| Mia Stewart | Dialogue Editor |
| Kaan Kalyon | Storyboard Artist |
| Ian McQue | Visual Development |
| Maarten Lemmens | Animation |
| Januel Mercado | Co-Director |
| Derek Cheung | Effects Supervisor |
| Gary Graham | Storyboard Artist |
| Joseph Butler | First Assistant Editor |
| Naz Ghodrati-Azadi | Storyboard Artist |
| Joseph Feinsilver | Art Direction |
| Jesús Alonso Iglesias | Character Designer |
| Brendan Kirschbaum | Animation |
| Lindsey Butterworth Langston | Animation |
| Emily Springer | Animation |
| Kelly Vawter | Animation |
| Anthony Holden | Storyboard Artist |
| Alice Herring | Storyboard Artist |
| Michael Lester | Storyboard Artist |
| Lyle Nagy | Storyboard Artist |
| Katie Parody | Assistant Editor |
| Randy Spahr | Assistant Editor |
| Brooke Shay Bradford | Animation |
| Lisa Briggs Sachs | Production Manager |
| Zachary Glynn | Effects Supervisor |
| Alvaro Michelena | Animation |
| Christopher Gonzalez | Animation |
| Christopher Campagna | Foley Editor |
| Adam Shaw | Foley Editor |
| Naveen Selvanathan | Visual Development |
| Daniel Cacouault | Visual Development |
| Wayne Tsay | Visual Development |
| Sébastian Piquet | Visual Development |
| Lori Chen | Production Assistant |
| Catherine Chooljian | Animation |
| John Han | Animation |
| Isaac Avital | Animation |
| Jun Hyuck Kim | Animation |
| Marceline Tanguay | Storyboard Artist |
| Danny Langston | Storyboard Artist |
| Dylan Stayman | Assistant Editor |
