 | Julia Roberts als Hova (voice) |
 | Meryl Streep als Queen (voice) |
 | Nicolas Cage als Zoc (voice) |
 | Paul Giamatti als Stan Beals (voice) |
 | Bruce Campbell als Fugax (voice) |
 | Regina King als Kreela (voice) |
 | Lily Tomlin als Mommo (voice) |
 | Larry Miller als Fred Nickle (voice) |
 | Cheri Oteri als Doreen Nickle (voice) |
 | Zach Tyler als Lucas Nickle (voice) |
 | Ricardo Montalban als Head of Council (voice) |
 | Myles Jeffrey als Steve (voice) |
 | Creagen Dow als Mullet Boy (voice) |
 | Frank Welker als Spindle / Frog / Caterpillar (voice) |
 | Mark DeCarlo als Fly (voice) |
 | Jake T. Austin als Nicky (voice) |
 | Allison Mack als Tiffany Nickle (voice) |
 | S. Scott Bullock als Glow Worm / Wasp Survivor (voice) |
 | Paul Greenberg als Sleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice) |
 | Rob Paulsen als Beetle (voice) |
 | Nicole Sullivan als Ant #9 (voice) |
 | Don Frye als Soldier Ant (voice) |
 | Larry Cedar als Ant #8 (voice) |
| Paul Greenberg als Sleeping Ant #1 / Head Lice #1 / Head Lice #2 (voice) |
 | David Kaye als Sleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice) |
| Alfred Jackson als Slacker Ant (voice) |
| Ken Mitchroney als Ant #17 / Guard Ant #2 / Wasp #8 (voice) |
 | Denzel Whitaker als Albert (voice) |
| Casey Masamitsu als Shigeko (voice) |
| Bryan Fabian als Fernando (voice) |
 | Jake Goldberg als Helmet Boy / Nob / Red Teammate #1 (voice) |
| Keith Alcorn als Ant #18 / Pupa / Soldier Ant #3 (voice) |
 | John A. Davis als Ant #19 (voice) |
 | Tom Kenny als Drone Ant / Ant #2 / Ant #6 (voice) |
| Sarah Mensinga als Mother Ant (voice) |
 | Nika Futterman als Ant #1 / Ant #7 (voice) |
| Scott Holst als Ant #3 (voice) |
| Susan Silo als Ant #4 (voice) |
 | Paul Rugg als Ant #5 (voice) |
 | Candi Milo als Nurse Ant #3 (voice) |
| Nissa Alcorn als Billo (voice) |
 | Pat Fraley als Ant Council #1 (voice) |
 | Tyler James Williams als Blue Teammate #1 (voice) |
| Jared Goldsmith als Blue Teammate #2 / Red Teammate #2 (voice) |
| Austin Majors als Blue Teammate #3 (voice) |
 | Jaishon Fisher als Blue Teammate #4 (voice) |
| Aaron Michael Drozin als Blue Teammate #5 (voice) |
 | Max Burkholder als Blue Teammate #6 (voice) |
| Sam Green als Red Teammate #3 (voice) |
 | Colin Ford als Red Teammate #4 (voice) |
 | Kendall Ryan Sanders als Red Teammate #5 (voice) |
 | Shane Baumel als Red Teammate #6 (voice) |
 | Neil Ross als Wasp #1 / Wasp #5 (voice) |
 | Bob Joles als Wasp #2 (voice) |
 | Wally Wingert als Wasp #3 (voice) |
| Leon Morenzie als Wasp #4 (voice) |
 | Jonathan David Cook als Wasp #6 (voice) |
 | Sean Donnellan als Soldier Ant #1 (voice) |
 | Tress MacNeille als Old Council Ant (voice) |
| Richard Green als Wasp Leader (voice) |
 | Clive Robertson als Hova's Wasp (voice) |
 | Vernee Watson-Johnson als Head Nurse (voice) |
| John A. Davis | Director |
| Keith Alcorn | Executive Producer |
| Diana Choi | Executive Producer |
| John Nickle | Author |
| William Fay | Executive Producer |
| Gary Goetzman | Producer |
| Tom Hanks | Producer |
| Scott Mednick | Executive Producer |
| Steven Shareshian | Executive Producer |
| Thomas Tull | Executive Producer |
| Ken Mitchroney | Director of Photography |
| Ruth Lambert | Casting |
| Jon Price | Editor |
| John Debney | Original Music Composer |
| Chris Consani | Art Direction |
| Barry E. Jackson | Production Design |
| John A. Davis | Producer |
| John A. Davis | Author |
| Johan Klingler | Art Department Manager |
| Barry E. Jackson | Production Manager |
| Kevin Kaska | Orchestrator |
| Alex Johns | Co-Executive Producer |
| Kim Saxon | Co-Producer |
| Angie Parks | Script Coordinator |
| Mark Gelfuso | Set Dresser |
| David Marcus | Assistant Sound Editor |
| Brian Risner | Dialogue Editor |
| David Lee Fein | Foley Artist |
| Willard Overstreet | Foley Editor |
| Shawn Murphy | Scoring Mixer |
| Mike Sawitzke | Sound |
| Val Kuklowsky | Sound Editor |
| Kim Benzine-Rowell | Production Manager |
| Lee Supercinski | Unit Production Manager |
| Tiffany Anne Brower | Special Effects Coordinator |
| Gregory Wade Reynolds | Special Effects Supervisor |
| Sean Jensen | CGI Supervisor |
| Timothy J. Peckham | Digital Compositor |
| Joe Beckley | Lighting Artist |
| Diane H. Newman | Script Supervisor |
| Greg Glaser | Systems Administrators & Support |
| Leisa McClelland | Art Department Coordinator |
| Roman Robbins | Lighting Technician |
| Amy Grieshaber | Animation Department Coordinator |
| Mark Thielen | Animation Supervisor |
| Rod Douglas | Set Dressing Artist |
| Chris DeLaGuardia | Color Timer |
| Kevin Connolly | Musician |
| Michael Jonascu | Sound Designer |
| Odin Benitez | Sound Effects Editor |
| Rick Kline | Sound Re-Recording Mixer |
| Eric Flickinger | Sound Recordist |
| Russell Calabrese | Storyboard Artist |
| Christopher T. Welch | Supervising Sound Editor |
| Jake Patton | First Assistant Editor |
| David Santiago | Visual Effects Supervisor |
| Charles B. Unger | Additional Editor |
| Ryan Heiferman | ADR Recordist |
| David Jobe | Foley Mixer |
| Scott Morgan | Foley Recordist |
| Solange S. Schwalbe | Foley Supervisor |
| Jeff Carson | Music Editor |
| David Tart | Animation Director |
| David Krentz | Character Designer |
| Troy Griffin | Layout Supervisor |
| Tom Grevera | Lead Animator |
| Barbara Harris | ADR Voice Casting |
| Nathan Schauf | Assistant Editor |
| Bret Marnell | Associate Editor |
| Jim Eberle | Digital Color Timer |
| Mo Henry | Negative Cutter |
| Stuart Michael Thomas | Music Programmer |
| Ken Mitchroney | Head of Story |
| Fred Gambino | Matte Painter |
| Zubin Wadia | Pipeline Technical Director |
| Autumn Baeza | Production Assistant |
| Michael B. Comet | Rigging Supervisor |
| Tanya A. Price | Travel Coordinator |
| William Stout | Conceptual Design |
| Michael Sean Foley | CG Supervisor |
| Armen Ksajikian | Musician |
| Eliott Taylor | Sound Recordist |
