 | Bob Newhart als Bernard (voice) |
 | Eva Gabor als Miss Bianca (voice) |
 | John Candy als Wilbur (voice) |
 | Tristan Rogers als Jake (voice) |
 | Adam Ryen als Cody (voice) |
 | George C. Scott als McLeach (voice) |
 | Wayne Robson als Frank (voice) |
 | Douglas Seale als Krebbs (voice) |
 | Frank Welker als Joanna (voice) |
 | Bernard Fox als Chairman / Doctor (voice) |
 | Peter Firth als Red (voice) |
 | Ed Gilbert als Francois (voice) |
| Carla Meyer als Faloo / Mother (voice) |
 | Russi Taylor als Nurse Mouse (voice) |
| Peter Greenwood als Radio Announcer / Airplane Captain (voice) |
 | Billy Barty als Baitmouse (voice) |
 | Mickie McGowan als International Mice (voice) (uncredited) |
| Mary V. Buck | Casting Director |
| Joe Ranft | Screenplay |
| Joe Ranft | Story Supervisor |
| Bruce Broughton | Original Music Composer |
| Karey Kirkpatrick | Screenplay |
| Mike Gabriel | Director |
| Margery Sharp | Characters |
| Jim Cox | Screenplay |
| Kathleen Gavin | Associate Producer |
| Hendel Butoy | Director |
| Pixote Hunt | Art Direction |
| Byron Simpson | Screenplay |
| Thomas Schumacher | Producer |
| Susan Edelman | Casting Director |
| Vera Pacheco | Animation |
| Michael Kelly | Editor |
| Bill Perkins | Layout |
| Anthony DeRosa | Animation |
| Johan Klingler | Animation |
| James Baxter | Animation |
| Scott F. Johnston | Visual Effects |
| Rob Bekuhrs | Special Effects |
| Larry White | Animation |
| Dan Perri | Title Designer |
