 | Matthew Broderick als Despereaux (voice) |
 | Dustin Hoffman als Roscuro (voice) |
 | Emma Watson als Princess Pea (voice) |
 | Tracey Ullman als Miggery Sow (voice) |
 | Kevin Kline als Chef Andre (voice) |
 | Sigourney Weaver als The Narrator (voice) |
 | William H. Macy als Lester (voice) |
 | Stanley Tucci als Boldo (voice) |
 | Ciarán Hinds als Botticelli (voice) |
 | Robbie Coltrane als Gregory (voice) |
 | Tony Hale als Furlough (voice) |
 | Frances Conroy als Antoinette (voice) |
 | Frank Langella als Mayor (voice) |
 | Richard Jenkins als Principal (voice) |
 | Christopher Lloyd als Hovis (voice) |
 | Charles Shaughnessy als Pietro (voice) |
 | Patricia Cullen als Queen (voice) |
 | McNally Sagal als Teacher (voice) |
 | Sam Fell als Ned / Smudge (voice) |
| Robert Stevenhagen | Director |
| Sam Fell | Director |
| Will McRobb | Screenstory |
| Chris Viscardi | Screenstory |
| Gary Ross | Producer |
| Allison Thomas | Producer |
| Kate DiCamillo | Book |
| Gary Ross | Screenplay |
| William Sargent | Executive Producer |
| Ryan Kavanaugh | Executive Producer |
| David Lipman | Executive Producer |
| Robin Bissell | Executive Producer |
| Tracy Shaw | Co-Producer |
| Evgeni Tomov | Production Design |
| Olivier Adam | Supervising Art Director |
| Gabriele Zucchelli | Animation Supervisor |
| Barry Armour | Visual Effects Supervisor |
| Brad Blackbourn | Director of Photography |
| Mark Solomon | Editor |
| Debra Zane | Casting |
| Nikolay Neykov | Character Designer |
| Evgeni Tomov | Character Designer |
| Tim Watts | Character Designer |
| Nelson Yokota | Character Designer |
| Claire Dodgson | Associate Editor |
| Aidan O'Brien | First Assistant Editor |
| Steve Pang | First Assistant Editor |
| Erika Gonzales | Assistant Editor |
| Chris Jenkins | Sound Re-Recording Mixer |
| Frank A. Montaño | Sound Re-Recording Mixer |
| Chris Jargo | ADR Supervisor |
| Celia Boydell | Associate Producer |
| Peiyu Foley | Associate Producer |
| Casey Crowe | Associate Producer |
| Jamal McLemore | Associate Producer |
| Tannis Vallely | Casting Associate |
| Tripp Hudson | Line Producer |
| Bart Boirot | Lead Animator |
| Rahul Dabholkar | Lead Animator |
| Phil Dale | Lead Animator |
| José Luis de Lucas | Lead Animator |
| Pete Dodd | Lead Animator |
| Matt Everitt | Lead Animator |
| Jeremy Lazare | Lead Animator |
| Michael Schlingmann | Lead Animator |
| Tim Watts | Lead Animator |
| Rob Woiwod | Assistant Editor |
| Jon Title | Sound Designer |
| Paul Aulicino | Sound Effects Editor |
| Anna MacKenzie | ADR Editor |
| Michelle Pazer | ADR Editor |
| Gayle Wesley | First Assistant Sound Editor |
| Cheryl Murphy | Post Production Supervisor |
| Caitlin McKenna | ADR Voice Casting |
| Kathy Nelson | Music Supervisor |
| Peter Myles | Music Editor |
| Conrad Pope | Orchestrator |
| William Ross | Orchestrator |
| Jerome Leroy | Orchestrator |
| Bruce Schluter | Title Designer |
| Terry Claborn | Color Timer |
| William Goldenberg | Thanks |
| Henry Bush | Pipeline Technical Director |
| William Ross | Original Music Composer |
| Lon Bender | Sound Designer |
| Lon Bender | Supervising Sound Editor |
