 | Colin Clive als Dr. Henry Frankenstein |
 | Mae Clarke als Elizabeth |
 | John Boles als Victor Moritz |
 | Boris Karloff als The Monster |
 | Edward Van Sloan als Dr. Waldman |
 | Frederick Kerr als Baron Frankenstein |
 | Dwight Frye als Fritz |
 | Lionel Belmore als Herr Vogel, the Burgomaster |
 | Marilyn Harris als Maria |
 | Ted Billings als Villager (uncredited) |
 | Mae Bruce als Screaming Maid (uncredited) |
 | Jack Curtis als Villager (uncredited) |
 | Arletta Duncan als Bridesmaid (uncredited) |
 | William Dyer als Gravedigger (uncredited) |
 | Francis Ford als Hans (uncredited) |
 | Soledad Jiménez als Mourner (uncredited) |
 | Carmencita Johnson als Little Girl (uncredited) |
| Seessel Anne Johnson als Little Girl (uncredited) |
 | Margaret Mann als Mourner (uncredited) |
 | Michael Mark als Ludwig (uncredited) |
 | Pauline Moore als Bridesmaid (uncredited) |
 | Inez Palange als Villager (uncredited) |
 | Paul Panzer als Mourner (uncredited) |
 | Cecilia Parker als Maid (uncredited) |
| Rose Plumer als Villager (uncredited) |
| Cecil Reynolds als Waldman's Secretary (uncredited) |
 | Ellinor Vanderveer als Medical Student (uncredited) |
 | Robert Milasch als Villager (uncredited) |
| James Whale | Director |
| Mary Shelley | Novel |
| Francis Edward Faragoh | Screenplay |
| Garrett Fort | Screenplay |
| Carl Laemmle Jr. | Producer |
| Arthur Edeson | Director of Photography |
| Clarence Kolster | Editor |
| Charles D. Hall | Art Direction |
| E.M. Asher | Associate Producer |
| C. Roy Hunter | Recording Supervision |
| Maurice Pivar | Supervising Film Editor |
| Carl Laemmle | Presenter |
| Richard Schayer | Script Editor |
| Peggy Webling | Theatre Play |
| Jack Pierce | Makeup Artist |
| Bernhard Kaun | Original Music Composer |
| Paul Ivano | Director of Photography |
| William Hedgcock | Sound Recordist |
| John P. Fulton | Special Effects |
| Robert Florey | Writer |
| John L. Balderston | Scenario Writer |
| John Russell | Screenplay |
| Jack Pierce | Makeup Designer |
| Pauline Eells | Wigmaker |
| Joseph A. McDonough | Assistant Director |
| Ed Keyes | Property Master |
| Herman Rosse | Set Designer |
| Franz Dallons | Props |
| Oscar Dallons | Props |
| Paul Dallons | Props |
| Ken Strickfaden | Special Effects |
| Cleo E. Baker | Visual Effects |
| Ken Strickfaden | Stunt Double |
| Sherman Clark | Still Photographer |
| Jack Eagan | Assistant Camera |
| Jack Freulich | Still Photographer |
| Alan Jones | Second Assistant Camera |
| George Trafton | Assistant Camera |
| Mae Bruce | Wardrobe Assistant |
| David Broekman | Music Director |
| Gilbert Kurland | Music Supervisor |
| Cecil Reynolds | Medical Consultant |
| Gerald L.G. Sampson | Technical Advisor |
| Jack Foley | Sound Effects |
