 | Charlie Tahan als Victor Frankenstein (voice) |
 | Catherine O'Hara als Mrs. Frankenstein / Weird Girl / Gym Teacher (voice) |
 | Martin Short als Mr. Frankenstein / Mr. Burgemeister / Nassor (voice) |
 | Martin Landau als Mr. Rzykruski (voice) |
 | Atticus Shaffer als Edgar "E" Gore (voice) |
 | Winona Ryder als Elsa Van Helsing (voice) |
 | Robert Capron als Bob (voice) |
 | James Hiroyuki Liao als Toshiaki (voice) |
 | Conchata Ferrell als Bob's Mom (voice) |
 | Tom Kenny als New Holland Townsfolk (voice) |
 | Frank Welker als Sparky Frankenstein (voice) |
 | Dee Bradley Baker als Persephone van Helsing / Shelly / Were-Rat / Colossus |
 | Jeff Bennett als Giant Sea Monkeys (voice) |
 | Christopher Lee als Movie Dracula (archive footage) |
| Ronna Kress | Casting |
| Rick Heinrichs | Production Design |
| Tim Burton | Director |
| Tim Burton | Producer |
| Tim Burton | Characters |
| Tim Burton | Idea |
| Danny Elfman | Original Music Composer |
| Chris Lebenzon | Editor |
| Christopher Boyes | Sound Re-Recording Mixer |
| John August | Screenplay |
| Tom Johnson | Sound Re-Recording Mixer |
| Michael Higham | Music Editor |
| Alexandra Walker | Art Direction |
| Shelley Duvall | Thanks |
| Andie Derrick | Foley Artist |
| Peter Burgis | Foley Artist |
| Daniel Stern | Thanks |
| Thomas L. Wilhite | Thanks |
| Don Hahn | Executive Producer |
| Allison Abbate | Producer |
| Jen Rudin | Local Casting |
| Barret Oliver | Thanks |
| Mark DeSimone | ADR Mixer |
| Leonard Ripps | Original Story |
| Julie Hickson | Thanks |
| Mark Solomon | Editor |
| Joan Schneider | Unit Production Manager |
| Jorgen Klubien | Storyboard |
| Derek Frey | Co-Producer |
| Stephen Perkins | Associate Editor |
| Cesar Diaz Melendez | Animation |
| Jessie Thiele | Post Production Supervisor |
| Peter Sorg | Director of Photography |
| Philip Vallentin | Storyboard |
| Andrew Bonner | Production Coordinator |
| Bjorn Ole Schroeder | Supervising Dialogue Editor |
| Michael Fentum | Sound Effects Designer |
| Jason Swanscott | Foley Artist |
| Glen Gathard | Foley Mixer |
| Steve Browell | Sound Effects Editor |
| Christian De Vita | Storyboard |
| Connie Nartonis Thompson | Associate Producer |
| Michael Semanick | Sound Re-Recording Mixer |
| Danail Kraev | Animation |
| Tobias Fouracre | Lead Animator |
| Oliver Tarney | Supervising Sound Editor |
| Dillon Bennett | Sound Effects Editor |
| Chris Gilligan | Animation |
| Padraig Collins | Storyboard |
| Tim Allen | Animation |
| Peter Dodd | Animation |
| Anthony Elworthy | Lead Animator |
| Jens Jonathan Gulliksen | Animation |
| Brian Leif Hansen | Lead Animator |
| Trey Thomas | Animation Director |
| Mark Waring | Animation Supervisor |
| Tim Ledbury | Visual Effects Supervisor |
| Daniel Alderson | Animation |
| Kristien Vanden Bussche | Animation |
| Rachelle Lambden | Animation |
| Matias Liebrecht | Animation |
| Chuck Duke | Animation |
| Kim Keukeleire | Animation |
| Jody Meredith | Animation |
| Marjolaine Parot | Animation |
| Dean Roberts | Storyboard |
| Sharon Smith | Storyboard |
| Steve Warne | Animation |
| Boris Wolf | Animation |
| Dobrin Yanev | Lead Animator |
| Gabriele Zucchelli | Storyboard |
| Rachael Tate | Assistant Sound Editor |
| Chris Navarro | ADR Mixer |
| Jeffrey Broom | Production Accountant |
| Edgardo Simone | Orchestrator |
| Tom Kemplen | First Assistant Editor |
| David Slonaker | Orchestrator |
| Simon Diggins | ADR Recordist |
| Richard Morrison | Title Designer |
| Dean Wares | Title Designer |
| Caitlin Well | Casting Associate |
| Simon Quinn | Line Producer |
| Kev Harwood | First Assistant Director |
| Tim Browning | Art Direction |
| Toby Farrar | Gaffer |
| Alice Lister | Production Coordinator |
| Faye Robinson | Production Coordinator |
| Richard Lake | Second Assistant Director |
| Jonny Ffinch | Visual Effects Producer |
| Lucy Benson | Visual Effects Editor |
