 | Sam Rockwell als Darwin the Guinea Pig (voice) |
 | Jon Favreau als Hurley the Guinea Pig (voice) |
 | Tracy Morgan als Blaster the Guinea Pig (voice) |
 | Penélope Cruz als Juarez the Guinea Pig (voice) |
 | Nicolas Cage als Speckles the Mole (voice) |
 | Dee Bradley Baker als Mooch the Fly (voice) |
 | Bill Nighy als Leonard Saber |
 | Will Arnett als Kip Killian |
 | Zach Galifianakis als Ben |
 | Kelli Garner als Marcie |
 | Steve Buscemi als Bucky the Hamster (voice) |
 | Jared Lavette als Jared the Skunk (voice) |
| Micah Lavette als Micah the Fox (voice) |
 | Niecy Nash-Betts als Rosalita |
 | Loudon Wainwright III als Grandpa Goodman |
 | Tyler Patrick Jones als Connor |
 | Piper Mackenzie Harris als Penny |
 | Chris Ellis als NSA Director |
 | Gabriel Casseus als Carter |
 | Justin Mentell als Terrell |
 | Troy Robinson als Agent |
 | Travis Davis als Agent |
| James Huang als Agent |
 | Corey Michael Eubanks als Agent |
 | Mini Anden als Saber's Assistant |
 | Camaron Engels als Skateboarder #1 (as Cameron Engels) |
 | Steve Kelso als Agent |
| Marianne Wibberley | Screenplay |
| Cormac Wibberley | Screenplay |
| Michael Leung | Lighting Artist |
| Justin Hammond | Technical Supervisor |
| Adam Holmes | Layout |
| Harald Kraut | Layout |
| Roger Huynh | Compositors |
| Roger Huynh | Lighting Artist |
| Brian Avery | Stunts |
| Brittany Petros | Production Manager |
| Diana R. Lupo | Stunts |
| Rick Marcus | Stunt Double |
| Lisa Curtis Saunders | Software Engineer |
| Jerry Bruckheimer | Producer |
| Mike Stenson | Executive Producer |
| Chad Oman | Executive Producer |
| Duncan Henderson | Executive Producer |
| David P.I. James | Executive Producer |
| Jason Hellmann | Editor |
| Mark Goldblatt | Editor |
| Ellen Mirojnick | Costume Design |
| Scott Stokdyk | Visual Effects Supervisor |
| Trevor Rabin | Original Music Composer |
| Kathy Nelson | Music Supervisor |
| Ronna Kress | Casting |
| Hoyt Yeatman | Story |
| David P.I. James | Story |
| Todd Arnow | Unit Production Manager |
| Richard Graves | First Assistant Director |
| Eric Sherman | Second Assistant Director |
| Pat Sandston | Associate Producer |
| Ted Elliott | Associate Producer |
| Terry Rossio | Associate Producer |
| Ed Kashiba | Associate Producer |
| John Clinton | Visual Effects Producer |
| Deborah La Mia Denaver | Makeup Department Head |
| Cinzia Zanetti | Makeup Artist |
| Peter Tothpal | Hair Department Head |
| Roxane Griffin | Hairstylist |
| Trudy Ramirez | Script Supervisor |
| Lee Orloff | Sound Mixer |
| Tami Goldman | Post Production Supervisor |
| George Watters II | Supervising Sound Editor |
| Terry Porter | Sound Re-Recording Mixer |
| Dean A. Zupancic | Sound Re-Recording Mixer |
| Jessica Gallavan | Supervising ADR Editor |
| Joe Dorn | ADR Editor |
| F. Hudson Miller | Sound Effects Editor |
| R.J. Palmer | Sound Effects Editor |
| Gary Wright | Sound Effects Editor |
| Adam Kopald | Sound Effects Editor |
| Randle Akerson | Sound Effects Editor |
| Shannon Mills | Sound Effects Designer |
| Lucy Coldsnow-Smith | Dialogue Editor |
| Victoria Martin | Foley Supervisor |
| David A. Arnold | Supervising Dialogue Editor |
| Matthew Harrison | Foley Editor |
| Thomas W. Small | Foley Editor |
| F. Scott Taylor | Assistant Sound Editor |
| Dee Selby | Assistant Sound Editor |
| Douglas Parker | Assistant Sound Editor |
| Dan O'Connell | Foley Artist |
| John T. Cucci | Foley Artist |
| James Ashwill | Foley Mixer |
| Barbara Harris | ADR Voice Casting |
| Doc Kane | ADR Mixer |
| David Boulton | ADR Mixer |
| Bobby Johanson | ADR Mixer |
| Charleen Richards-Steeves | ADR Mixer |
| Amy Denning Winfrey | Payroll Accountant |
| Michael Singer | Unit Publicist |
| Mauriece Jacks Jr. | Video Assist Operator |
| Russell McEntyre | Transportation Coordinator |
| Mike Padovich | Transportation Captain |
| Courtney Bright | Casting Associate |
| Tammy L. Smith | Extras Casting |
| Nicole Daniels | Casting Assistant |
| Jeanette Surga | Supervising Music Editor |
| Katie Greathouse | Music Editor |
| Robb Boyd | Music Editor |
| Kurt Smith | Color Timer |
| Jim Passon | Color Timer |
| Cheryl Cravedi | Set Costumer |
| Daniel R. Jennings | Art Direction |
| Steve Callas | Construction Coordinator |
| Robert L. Thompson | Construction Foreman |
| Deborah Evans | Production Design |
| Emily Ferry | Property Master |
| Leslie E. Rollins | Set Decoration |
| Ramsey Avery | Supervising Art Director |
| Robert Dawson | Title Designer |
| Patrick Loungway | Camera Operator |
| Bojan Bazelli | Director of Photography |
| Robert Zuckerman | Still Photographer |
| Hoyt Yeatman | Director |
| Shauna Duggins | Stunt Double |
| Armen Ksajikian | Musician |
| Troy Robinson | Stunts |
| Doug Coleman | Stunt Coordinator |
| Whitney Coleman | Stunts |
| Gregory J. Barnett | Stunts |
| Chris Palermo | Stunts |
| Jimmy N. Roberts | Utility Stunts |
| Alicia Vela-Bailey | Utility Stunts |
| Clay Cullen | Stunts |
