 | Ray Romano als Manny (voice) |
 | John Leguizamo als Sid (voice) |
 | Denis Leary als Diego (voice) |
 | Queen Latifah als Ellie (voice) |
 | Seann William Scott als Crash (voice) |
 | Josh Peck als Eddie (voice) |
 | Jay Leno als Fast Tony (voice) |
 | Will Arnett als Lone Gunslinger Vulture (voice) |
 | Chris Wedge als Scrat (voice) |
 | Peter Ackerman als Dung Beetle Dad (voice) |
| Caitlin Rose Anderson als Glypto Boy Billy / Beaver Girl (voice) |
| Connor Anderson als Rhino Boy / Beaver Boy (voice) |
 | Joseph Bologna als Mr. Start (voice) |
| Jack Crocicchia als Elk Boy (voice) |
| Peter DeSève als Condor Chick (voice) |
 | Ariel Winter als Additional Voices (voice) |
 | Clea Lewis als Female Mini Sloth (voice) |
 | Stephen Root als Aardvark Dad (voice) |
| Nicole DeFelice als Start Girl (voice) |
 | Debi Derryberry als Diatryma Mom (voice) |
 | Marshall Efron als Start Dad (voice) |
 | Tom Fahn als Stu / Male Ox (voice) |
| Jason Fricchione als Molehog Grandpa / Shovelmouth Male (voice) |
| James Edmund Godwin als Traffic Vulture (voice) |
| George Jacobs als Beaver Dad (voice) |
 | Brian Scott McFadden als Glyptodon (voice) |
 | Jansen Panettiere als Shovelmouth Boy (voice) |
| Gregory Romano als Start Boy 1 (voice) |
 | Matt Romano als Start Boy 2 (voice) |
 | Carlos Saldanha als Dodo (voice) |
| Manoela Scarpa Saldanha als Molehog Kid 1 (voice) |
| Sofia Scarpa Saldanha als Molehog Kid 2 (voice) |
| Reyna Shaskan als Diego's Bird Girl (voice) |
 | James Sie als Freaky Male (voice) |
| Cindy Slattery als Aardvark Mom (voice) |
 | Mindy Sterling als Female Ox (voice) |
 | Renée Taylor als Mrs. Start (voice) |
 | Alan Tudyk als Cholly (voice) |
 | Claudia Besso als Additional Voices (voice) |
 | Madeleine Martin als Additional Voices (voice) |
 | Sherry Lynn als Additional Voices (voice) |
 | Mickie McGowan als Additional Voices (voice) |
| Emily Anderson als Additional Voices (voice) |
 | Jack Angel als Additional Voices (voice) |
 | Shane Baumel als Additional Voices (voice) |
 | Bob Bergen als Additional Voices (voice) |
| Kyle J. Bogert als Additional Voices (voice) |
 | Rodger Bumpass als Additional Voices (voice) |
 | Paul Butcher als Additional Voices (voice) |
 | Blanca Camacho als Additional Voices (voice) |
 | John Cygan als Additional Voices (voice) |
 | Jennifer Darling als Additional Voices (voice) |
 | Olivia DeLaurentis als Additional Voices (voice) |
 | Harrison Fahn als Additional Voices (voice) |
 | Bill Farmer als Additional Voices (voice) |
 | Crispin Freeman als Additional Voices (voice) |
| Tim Kirkpatrick als Additional Voices (voice) |
| David H. Kramer als Additional Voices (voice) |
 | Danny Mann als Additional Voices (voice) |
 | Laura Marano als Additional Voices (voice) |
| Matt McCarthy als Additional Voices (voice) |
 | Laraine Newman als Additional Voices (voice) |
 | Jan Rabson als Additional Voices (voice) |
 | Zack Shada als Additional Voices (voice) |
 | Thomas Sharkey als Additional Voices (voice) |
 | Ross Simanteris als Additional Voices (voice) |
| Rachel Stand als Additional Voices (voice) |
 | Spencer Lacey Ganus als Additional Voices (voice) |
 | Jess Harnell als Additional Voices (voice) |
| Alex Sullivan als James (voice) |
| Carlos Saldanha | Director |
| Jon Vitti | Screenplay |
| John Powell | Original Music Composer |
| Yujin Ito | Visual Effects |
| Ben Sanders | Visual Effects |
| Lori Forte | Producer |
| Arun Ram-Mohan | Lighting Artist |
| Jeannine Berger | Post Production Supervisor |
| Bob Gordon | Line Producer |
| Chris Meledandri | Executive Producer |
| Chris Wedge | Executive Producer |
| Will Edwards | Original Music Composer |
| Craig Grasso | Director of Photography |
| Harry Hitner | Editor |
| Christian Kaplan | Casting |
| Thomas Cardone | Art Direction |
| Bruce Anderson | Production Manager |
| Samantha Berg | Assistant Production Manager |
| Jason Fricchione | Assistant Production Manager |
| Ilyssa Katz | Assistant Production Manager |
| Angela Macias | Assistant Production Manager |
| Meredith McIvor | Assistant Production Manager |
| Sean M. Murphy | Assistant Production Manager |
| Anthony Nisi | Production Supervisor |
| Andrea Blasich | Sculptor |
| Michael J. Travers | CG Supervisor |
| Peter Clarke | Conceptual Design |
| Robert MacKenzie | Color Designer |
| Kane Lee | Schedule Coordinator |
| Shawn Murphy | Scoring Mixer |
| Daniel Patrick O'Brien | Sequence Leads |
| Haji Uesato | Sequence Leads |
| Lora Hirschberg | Sound Mixer |
| Chris Scarabosio | Sound Mixer |
| Morten Folmer Nielsen | ADR Editor |
| Galen T. Chu | Animation Supervisor |
| James Bresnahan | Animation Supervisor |
| James Campbell | Animation |
| Amber Rudolph | Animation |
| Scott Carroll | Animation |
| Shaun Cusick | Sculptor |
| Eldar Cholich | Digital Compositors |
| Dann Tarmy | Digital Compositors |
| Ari Rubenstein | Digital Compositors |
| Dan Cayer | Digital Compositors |
| Sue Fox | Foley Editor |
| Mike Defeo | Sculptor |
| Monika Sawyer | Visual Effects Technical Director |
| Brian P. Dean | Lighting Technician |
| Thomas A. Carlson | Music Editor |
| James Thatcher | Musician |
| Jinyi Baird | Lighting Coordinator |
| Brett Hoffman | Script Coordinator |
| Randy Thom | Sound Designer |
| Randy Thom | Supervising Sound Editor |
| Randy Thom | Sound Mixer |
| David C. Hughes | Sound Effects Editor |
| Daniel Lopez Muñoz | Visual Development |
| Michael Knapp | Visual Development |
| Gareth Porter | Lead Set Dresser |
| James Willingham III | Set Dresser |
| James Parente | Set Dresser |
| Mike Thurmeier | Supervising Animator |
| Joe Bonomo | Systems Administrators & Support |
| Jayme Wilkinson | Technical Supervisor |
| Erin Crackel | Assistant Editor |
| Craig Paulsen | Associate Editor |
| Jim Passon | Color Timer |
| Randy Trager | First Assistant Editor |
| Gary Burritt | Negative Cutter |
| David Esneault | Lighting Supervisor |
| Luke Martorelli | Master Lighting Artist |
| Mark Piretti | Rigging Supervisor |
| Stuart McCowan | ADR Editor |
| Marshall Winn | ADR Editor |
| David Acord | Assistant Sound Editor |
| Cheryl Nardi | Assistant Sound Editor |
| Pete Anthony | Conductor |
| Dennie Thorpe | Foley |
| Jana Vance | Foley |
| Samuel H. Hinckley | Foley Editor |
| George Doering | Musician |
| Brad Dechter | Orchestrator |
| Bruce Fowler | Orchestrator |
| Randy Kerber | Orchestrator |
| Mark McKenzie | Orchestrator |
| Al Nelson | Sound Effects Editor |
| Will Edwards | Sound Recordist |
| Greg Farrell | Sound Recordist |
| Douglas Murray | Supervising Sound Editor |
| Gwendolyn Yates Whittle | Supervising Sound Editor |
| Patrick Danaher | Animation |
| Alan Rogers | Animation |
| Nick Bruno | Animation |
| Raul Chavez | Animation |
| Kevin Chesnos | Animation |
| Jerod Chirico | Animation |
| Jonathen Collins | Animation |
| David Couchariere | Animation |
| Ryan Denniston | Animation |
| Paul Downs | Animation |
| Jeff Gabor | Animation |
| David Gallagher | Animation |
| Leif Jeffers | Animation |
| Eddie Kim | Animation |
| Moonsung Lee | Animation |
| Jean Lin | Animation |
| Chip Lotierzo | Animation |
| Robin Luera | Animation |
| Jason Martinsen | Animation |
| Rich McKain | Animation |
| P. Jeep Naarkom | Animation |
| Pete Paquette | Animation |
| David S. Peng | Animation |
| Antonin Plante | Animation |
| Robyne Powell | Animation |
| Andreas Procopiou | Animation |
| Mika Ripatti | Animation |
| Nickolas Rosario | Animation |
| Derek Rozmes | Animation |
| Chad Sellers | Animation |
| Jeremy Shaw | Animation |
| Stephen L. Wong | Animation |
| Peter DeSève | Character Designer |
| Jason Davies | Creature Technical Director |
| Aaron Hartline | Lead Animator |
| David Torres | Lead Animator |
| Royce Wesley | Lead Animator |
| Ian M. Burkard | Modeling |
| Brien Hindman | Modeling |
| Edward Robbins | Modeling |
| Motoko Wada | Modeling |
| Daniel Williams | Modeling |
| Jon Campbell | Visual Effects |
| John A. Patterson | Visual Effects |
| Baaron Schulte | VFX Artist |
| Peter Gaulke | Screenplay |
| Gerry Swallow | Screenplay |
| Jim Hecht | Screenplay |
| Peter Gaulke | Story |
| Gerry Swallow | Story |
| Brandon Proctor | Sound Mix Technician |
| Jamie Williams | Visual Effects Technical Director |
| Moroni Taylor | Storyboard Artist |
| Will Files | Assistant Sound Designer |
| Tamir Sammy Diab | Compositor |
| Bryan Useo | Modeling |
| Nash Dunnigan | Layout |
| Natasha Leonnet | Digital Intermediate |
| James McKee Smith | Additional Music |
| Steve Moore | Storyboard |
| Christi Bertelsen | Storyboard Assistant |
| Derek Casari | ADR Engineer |
| Troy Porter | ADR Mixer |
| Roy Latham | ADR Recordist |
| Ellen Heuer | Foley Artist |
| Frank Rinella | Foley Mixer |
| Sean England | Foley Recordist |
| Gwendolyn Yates Whittle | Supervising ADR Editor |
| Monique Donnelly | Vocals |
| Hans Dastrup | Senior Animator |
| Tim Nordquist | Co-Editor |
| Amy Pawlowski | Digital Intermediate Editor |
| Robert V. Cavaleri | Effects Supervisor |
| Brian A. Keane | General Manager |
| Christopher Kappler | Human Resources |
| Justin Mandel | Production Assistant |
| Allen Dam | Production Consultant |
| Bob Camp | Story Artist |
| Robert Cardone | Layout Supervisor |
