 | Robbie Daymond als Spider-Man / Security Captain / Doc Ock (voice) |
 | Ben Pronsky als Venom / Security Guard #2 / Delivery guy (voice) |
 | Josh Keaton als Green Goblin / Police Officer / Security Guard #1 (voice) |
 | Tara Strong als Mary Jane Watson / Scientist #2 (voice) |
 | Jennifer Hale als Science Teacher / Scientist #1 (voice) |
 | Laura Bailey als Gwen Stacy / Ghost-Spider / Child (voice) |
| Eugene Son | Writer |
| Ken Cunningham | Director |
| Stan Lee | Characters |
| Steve Ditko | Characters |
| Andrew Duncan | Director |
