 | James Whitmore als Mark Twain (voice) |
 | Michele Mariana als Becky Thatcher (voice) |
 | Gary Krug als Huck Finn (voice) |
| Chris Ritchie als Tom Sawyer (voice) |
| John Morrison als Adam (voice) |
| Carol Edelman als Eve (voice) |
| Dal McKennon als Jim Smiley (voice) |
| Herb Smith als The Stranger (voice) |
| Marley Stone als Aunt Polly (voice) |
| Wilbur Vincent als The Mysterious Stranger (voice) |
| Wally Newman als Captain Stormfield (voice) |
| Billy Scream als The Indexivator (voice) |
 | Will Vinton als Dan'l Webster (voice) |
| Tom Gasek als Homer (voice) |
| Susan Shadburne | Writer |
| Will Vinton | Director |
| Kelley Baker | Sound Effects Editor |
| Kelley Baker | Sound Effects Designer |
| Kelley Baker | Negative Cutter |
| Kelley Baker | Editor |
| Mark Twain | Story |
| Billy Scream | Original Music Composer |
| Barry Bruce | Character Designer |
| Hugh Kennedy Tirrell | Executive Producer |
| Joan C. Gratz | Set Designer |
| Don Merkt | Set Designer |
| Bill Feisterman | Animation |
| Tom Gasek | Animation |
| Mark Gustafson | Animation |
| Barry Bruce | Animation |
| Craig Bartlett | Animation |
| Bruce McKean | Animation |
| Don Merkt | Animation |
| Will Vinton | Animation |
| Matt Wuerker | Animation |
