 | Ben Stiller als Larry Daley |
 | Carla Gugino als Rebecca |
 | Dick Van Dyke als Cecil |
 | Mickey Rooney als Gus |
 | Bill Cobbs als Reginald |
 | Jake Cherry als Nick Daley |
 | Ricky Gervais als Dr. McPhee |
 | Robin Williams als Teddy Roosevelt |
 | Kim Raver als Erica Daley |
 | Patrick Gallagher als Attila the Hun |
 | Rami Malek als Ahkmenrah |
 | Pierfrancesco Favino als Christopher Columbus |
 | Charlie Murphy als Taxi Driver |
 | Steve Coogan als Octavius |
 | Mizuo Peck als Sacajawea |
 | Kerry van der Griend als Neanderthal #1 |
 | Dan Rizzuto als Neanderthal #2 |
 | Matthew Harrison als Neanderthal #3 |
 | Jody Racicot als Neanderthal #4 |
 | Paul Rudd als Don |
 | Anne Meara als Debbie |
 | Martin Christopher als Lewis |
 | Martin Sims als Clark |
 | Randy Lee als Hun #1 |
 | Darryl Quon als Hun #2 |
| Gerald Wong als Hun #3 |
 | Paul Chih-Ping Cheng als Hun #4 |
 | Teagle F. Bougere als Teacher (Mike) |
 | Pat Kiernan als TV News Anchor |
 | Nico McEown als Friend #1 |
| Meshach Peters als Friend #2 |
 | Matthew Walker als Politician |
 | Jason McKinnon als Irish Worker |
| Jono Lee als Chinese Rail Worker |
 | Jason Vaisvila als Viking |
| Cade Wagar als Viking |
| Cory Martin als Chinese Terra Cota Soldier |
 | Brad Garrett als Easter Island Head (voice) |
 | Crystal the Monkey als Dexter |
 | Che Landon als Pretty Tourist Girl (uncredited) |
 | Phil Proctor als Moose (uncredited) |
 | Gary Sievers als Viking Warrior (uncredited) |
 | Owen Wilson als Jedediah Smith (uncredited) |
| Alan Silvestri | Original Music Composer |
| Thomas Lennon | Screenplay |
| Guillermo Navarro | Director of Photography |
| Renée April | Costume Design |
| Ilene Starger | Casting |
| Josh McLaglen | Co-Producer |
| Josh McLaglen | First Assistant Director |
| Michele Laliberte | Art Direction |
| Chris Columbus | Producer |
| Mark Radcliffe | Executive Producer |
| Michael Diner | Art Direction |
| Lin MacDonald | Set Decoration |
| Don Zimmerman | Editor |
| Brian Smrz | Second Unit Director |
| Shawn Levy | Director |
| Shawn Levy | Producer |
| Michael Barnathan | Producer |
| Claude Paré | Production Design |
| Bridget McGuire | Art Direction |
| Grant Van Der Slagt | Art Direction |
| Karen Patch | Wardrobe Designer |
| Larry Blanford | Second Unit Director of Photography |
| Helen Jarvis | Supervising Art Director |
| R.J. Kizer | ADR Supervisor |
| Victoria Down | Makeup Artist |
| Tim Gomillion | Sound Recordist |
| Warren Hendriks | First Assistant Sound Editor |
| Mildred Iatrou | ADR Editor |
| David Lucarelli | ADR Recordist |
| John Murray | Foley Supervisor |
| Charleen Richards | ADR Mixer |
| Dennis Rogers | Sound Recordist |
| Kimberly Chiang | Stunts |
| Thomas M. Hammel | Executive Producer |
| Thomas M. Hammel | Unit Production Manager |
| Ben Garant | Screenplay |
| Marty Eli Schwartz | First Assistant Director |
| Ira Shuman | Executive Producer |
| Ira Shuman | Unit Production Manager |
| Milan Trenc | Book |
| Stefán Karl Stefánsson | Other |
| Blaise Corrigan | Stunt Driver |
| Chris Beach | Set Designer |
| Chris Regan | Color Timer |
| J.J. Makaro | Stunt Coordinator |
| Armen Ksajikian | Musician |
| Glenn Bydwell | Set Designer |
| Craig Ricci Shaynak | ADR & Dubbing |
| William H. Orr | Special Effects Coordinator |
| Blanche Boileau | Costume Supervisor |
| Peter Bodnarus | Set Designer |
| Dan O'Connell | Foley Artist |
| Craig Henighan | Sound Designer |
| Craig Henighan | Supervising Sound Editor |
| John T. Cucci | Foley Artist |
| David Crone | "A" Camera Operator |
| Susan Dawes | Dialogue Editor |
| Dave Kulczycki | Sound Effects Editor |
| Doug Girling | Set Designer |
| John A. Larsen | Supervising Sound Editor |
| Matt Patterson | Sound Recordist |
| Gloria Davies | Unit Publicist |
| Terry Wilson | Supervising Music Editor |
| Jan Kobylka | Construction Coordinator |
| Donald Sylvester | Foley Editor |
| Ellen Freund | Property Master |
| Sherry Linder-Gygli | Hairstylist |
| Rob Vreugde | Transportation Coordinator |
| Jim Rygiel | Visual Effects Supervisor |
| Glen A. Dickson | "B" Camera Operator |
| Skip Longfellow | Assistant Sound Editor |
| Julie D'Antoni | Visual Effects Producer |
| Ellen Somers | Associate Producer |
| Diane Durant | Script Supervisor |
| Zoe E. Rotter | Casting Associate |
| Rob Young | Production Sound Mixer |
| Helen Luttrell | Dialogue Editor |
| James Ashwill | Foley Mixer |
| John Dale | Construction Foreman |
| Daniel Mansfield | Second Second Assistant Director |
| Michael Hibberson | Boom Operator |
| Neil Robertson | Location Manager |
| Gary Burritt | Negative Cutter |
| Misha Bukowski | Second Assistant Director |
| Valerie Lindquist | Casting Assistant |
| James Salberg | Dolly Grip |
| Loree Cameron | Graphic Designer |
| Ray Lai | Graphic Designer |
| Rhonda Taylor | Third Assistant Director |
| James Forsyth | Extras Casting |
| Scott Cozens | Camera Loader |
| Neil McBride | Dolly Grip |
| Anne M. Simonet | Unit Production Manager |
