 | Freddie Prinze Jr. als Fred Jones |
 | Sarah Michelle Gellar als Daphne Blake |
 | Matthew Lillard als Norville "Shaggy" Rogers |
 | Linda Cardellini als Velma Dinkley |
 | Seth Green als Patrick Wisely |
 | Peter Boyle als Old Man Wickles |
 | Tim Blake Nelson als Johnathan Jacobo |
 | Alicia Silverstone als Heather Jasper-Howe |
 | Kevin Durand als Black Knight Ghost |
 | Neil Fanning als Scooby-Doo (voice) |
 | Bill Meilen als Chauffeur |
 | Chris Gauthier als Daphne's Tattooed Fan #1 |
 | Peter New als Daphne's Tattooed Fan #2 |
 | Calum Worthy als Kid on Bike #2 |
 | Colin Foo als Investor |
 | Pat O'Brien als Pat O'Brien |
 | Zahf Paroo als Ned |
 | C. Ernst Harth als Miner 49er |
 | Stephen E. Miller als C.L. Magnus |
| Ted Kozma als Museum Guard |
 | Lisa Ann Beley als Mullet Nerdette #2 |
| Scott McNeil als Evil Masked Figure (voice) |
| Ruben Studdard als Ruben Studdard |
 | Bob Papenbrook als Black Knight Ghost (voice) |
 | Dee Bradley Baker als 10,000 Volt Ghost / Zombie / Red Eye Skeleton (voice) |
 | Michael Sorich als Tar Monster / Cotton Candy Glob (voice) (as Michael J. Sorich) |
 | Terrence Stone als 10,000 Volt Ghost (voice) |
 | Wally Wingert als Green-Eyed Skeleton (voice) |
 | J.P. Manoux als Brainiac Scooby (voice) |
 | Brandon Jay McLaren als Skater Dude #2 |
 | Michael Buffer als (voice) |
 | Emily Tennant als Young Daphne |
 | Tiffani Timms als Reporter #4 |
 | Alexz Johnson als Fred Fan |
 | Morgan Brayton als Mullet Nerdette #1 |
 | Nazanin Afshin-Jam als Shaggy Chick (uncredited) |
| Charles Roven | Producer |
| Raja Gosnell | Director |
| Mary Vernieu | Casting |
| David Newman | Original Music Composer |
| David Newman | Conductor |
| Shepherd Frankel | Supervising Art Director |
| Oliver Wood | Director of Photography |
| Alan Glazer | Co-Producer |
| Jeff Carson | Supervising Music Editor |
| Anne Fletcher | Choreographer |
| Kent Beyda | Editor |
| Joseph Barbera | Executive Producer |
| Joseph Barbera | Characters |
| William Hanna | Characters |
| James Gunn | Writer |
| James Gunn | Co-Producer |
| Andy Armstrong | Stunt Coordinator |
| Andy Armstrong | Second Assistant Director |
| Adam Shankman | Choreographer |
| Gitte Axen | Makeup Artist |
| Leesa Evans | Costume Design |
| Joanna Moore | Second Second Assistant Director |
| Tedd Kuchera | Set Decoration |
| Bill Terezakis | Prosthetic Supervisor |
| Brent O'Connor | Executive Producer |
| Brent O'Connor | Unit Production Manager |
| James Bamford | Stunts |
| Melissa R. Stubbs | Stunts |
| Kelley Smith-Wait | Executive Producer |
| Richard Suckle | Producer |
| John Alvarez | Assistant Art Director |
| Michael N. Wong | Art Direction |
| Michael Buffer | Musician |
| Ashlea Earl | Stunts |
| Darren Higman | Music Supervisor |
| Kelly Zombor | Boom Operator |
| Nerses Gezalyan | Foley Mixer |
| Kimaree Long | Dialogue Editor |
| James Moriana | Foley Artist |
| Lance Johnson | Property Master |
| Anthony Harris | Digital Color Timer |
| Don MacAulay | Art Direction |
| J.J. Makaro | Stunt Coordinator |
| Kerry Ann Carmean | Sound Effects Editor |
| Doug Chapman | Stunts |
| Bill Boes | Production Design |
| Mark Kenna | Dolby Consultant |
| Anji Bemben | Hairstylist |
| Daniel Arkin | Visual Effects Editor |
| Jim Filippone | Helicopter Camera |
| Jim Filippone | Pilot |
| Charles Lyall | Production Manager |
| Markus Czyzewski | Music Editor |
| Crystal Dalman | Stunts |
| Matt Diezel | Assistant Editor |
| Jacques Levesque | Compositing Supervisor |
| Richard Cowan | Associate Producer |
| Richard Cowan | First Assistant Director |
| Nesta Chapman | Stunts |
| Brian Shell | Construction Coordinator |
| Peter Ochotta | Art Department Assistant |
| Kristin Solid | Animation |
| Steven J. Winslow | Camera Technician |
| Diyah Pera | Still Photographer |
| Brent Gloeckler | Sculptor |
| Stacey Butterworth | Wig Designer |
| Ron Barr | Digital Intermediate Editor |
| Lindsay Hallett | Visual Effects Production Manager |
| Constantine Sekeris | Conceptual Design |
| Perry Battista | Lead Set Dresser |
| Luisa Dalmagro | Set Costumer |
| Julia Frittaion | Publicist |
| Erika A. McKee | Digital Producer |
| Laura Harris Atkinson | Supervising Dialogue Editor |
| Chris Hogan | Sound Editor |
| Todd Shifflett | Digital Effects Supervisor |
| Rick Rasmussen | Picture Car Coordinator |
| Jim Van Dijk | First Assistant "A" Camera |
| Dan Mannix | Steadicam Operator |
| Raymond Chen | Lighting Supervisor |
| Stephanie Allen | Visual Effects Producer |
| Jason Piccioni | Digital Supervisor |
| John MacCarthy | Second Unit First Assistant Director |
| Rif Dagher | Researcher |
| Valance Eisleben | Editorial Services |
| Michael Shand | CGI Supervisor |
| Scott Wolf | Sound Designer |
| Laura Boddington | Stand In |
| Conrad Dueck | 3D Supervisor |
| Michael D. Wilhoit | Supervising Sound Editor |
| Jim Dunn | Stunt Double |
| Kelvin Lee | Lead Animator |
| Mira Caveno | Set Designer |
| Jason Crosby | Marine Coordinator |
| Joey Lessard | CG Supervisor |
| Glen A. Dickson | Additional Camera |
| Cydney Cornell | Hairdresser |
| Hugo Dominguez | Visual Effects |
| Angie Jones | Animation |
| Kirk Johns | Location Manager |
| Bill Haller | Supervising Animator |
| Andrew Arnett | Animation |
| Steeve Boulianne | Layout |
| Steve Cady | Animation |
| Jeff Croke | Animation |
| Brian Dowrick | Animation |
| Steve Harwood | Animation |
| Chris Lentz | Animation |
| Leigh H. Rens | Animation |
| Matt Williames | Animation |
| Tim Arasheben | Helicopter Camera |
| Daniel R. Chavez | Executive In Charge Of Post Production |
| Mitch Gates | Visual Effects Technical Director |
| Kelly Oxford | ADR Editor |
| Daphne Klebe | Art Department Coordinator |
| David Allinson | Special Effects Coordinator |
| Dennis Brock | Chief Lighting Technician |
| Diana Stata | Assistant Music Supervisor |
| Bill Draper | Executive In Charge Of Production |
| Benton Jew | Creature Design |
| Tim Hogan | Key Grip |
| Jeff Glueck | Sound Engineer |
| Alison Fraser | Costume Coordinator |
| Errin Lally | Casting Coordinator |
| David Husby | Production Sound Mixer |
| Sandes Ashe | First Assistant Camera |
| Steve Ellis | Transportation Captain |
| Herb DeWaal | Key Rigging Grip |
| Tony Swatton | Armorer |
| Gregory Jamrok | Orchestrator |
| Whitney Kitchen | Animation Coordinator |
| Chris Bond | Visual Effects Supervisor |
| Christopher Charles Kempinski | Lighting Technician |
| Keith Hunter | Modeling |
| Warren Dunlop | Construction Foreman |
| Brad Oleksy | Greensman |
| Rick Allen | Grip |
| Patrick Booker | Carpenter |
| Dwane McIsaac | Chef |
| Jim Rankin | Craft Service |
| Ray Evans | Driver |
| Fernando Favila | Makeup Effects |
| Joan Kelley Bierman | Post Production Supervisor |
| Chris Bradford | Security |
| Ivan Neulander | Software Engineer |
| Nick Allen | Stunts |
| Steve Linn | Systems Administrators & Support |
| László Sebõ | Technical Supervisor |
| Peter Huff | Transportation Coordinator |
| Cody Laudan | Utility Stunts |
| Ronald Schlueter | Video Assist Operator |
| Ronnie Baran | Best Boy Electric |
| Tim Atkins | Gaffer |
| John Lai | Lighting Artist |
| Cory Hodson | Rigging Gaffer |
| Mandy Butler | Production Accountant |
| Juliet Verni | Production Coordinator |
| David Kudell | Assistant Sound Editor |
| Mathieu Blouin | Animation Supervisor |
| Laide Agunbiade | Digital Compositors |
| David Zbriger | I/O Supervisor |
| Mike Splatt | Special Effects Supervisor |
| August Hall | Visual Development |
| Melany Bourgouin | Visual Effects Coordinator |
| Brom | Storyboard Artist |
| Karen Schell | Sound |
| Jeff Hall | Assistant Chief Lighting Technician |
| Mike Larivee | Special Effects Technician |
| David Klohn | Second Assistant Director |
| Mo Henry | Negative Cutter |
| Misha Bukowski | Third Assistant Director |
| Tom Boyd | Musician |
| Barrie Wells | Techno Crane Operator |
| James McKenzie Moore | Casting Assistant |
| Cheryl Budgett | 2D Supervisor |
| Santino Barile | Assistant Property Master |
| Tracy B. Dunlop | Set Dresser |
| Todd Hlagi | Dolly Grip |
| Jody Chow | Assistant Production Coordinator |
| Adam de Bosch Kemper | Pre-Visualization Supervisor |
| Eric Gotthelf | ADR Mixer |
| Lionel Barton | Colorist |
| Stephanie Boeke | Extras Casting |
| Jim Bacon | Epk Producer |
| Jay Anderson | Generator Operator |
| Suzie Bruce | Assistant Accountant |
| Michael Lemmers | Best Boy Grip |
| Rob Miller | Contact Lens Technician |
| Tamar Ouziel | Assistant Makeup Artist |
| Jina Johnson | Assistant Location Manager |
| Marta Knudsen | Matte Painter |
| Shaun Bullied | Set Painter |
| Elizabeth Beauchamp | Costumer |
| Leah Hong | Graphic Designer |
| Sean Lavoie | Paint Coordinator |
| Darya Douglas-Andres | Special Effects Assistant |
| Harold Bernard | Second Assistant Camera |
| James Bluma | Assistant Editor |
| Will Cunningham | Compositing Artist |
| Barbara Copp | Key Production Assistant |
| Jim Bach | Playback Coordinator |
| Eve-Marie Gingras | Production Supervisor |
| Jamie Reid Tait | Second Assistant Production Coordinator |
| Richard Brooks | Stunts |
| Alette Falle | Stunts |
