 | Frank Welker als Scooby-Doo / Fred Jones (voice) |
 | Grey DeLisle als Daphne Blake / Wonder Woman (voice) |
 | Matthew Lillard als Shaggy Rogers (voice) |
 | Kate Micucci als Velma Dinkley (voice) |
 | P.J. Byrne als J.B. (voice) |
 | Victoria Grace als Mercy (voice) |
 | Charles Halford als Lex Luthor (voice) |
 | Nolan North als Computer / Joker / Superman (voice) |
 | Tara Strong als Helen / Lois Lane / Harley Quinn (voice) |
 | Fred Tatasciore als Solomon Grundy / Perry White (voice) |
 | James Arnold Taylor als Jimmy Olsen / Rex Ruthor (voice) |
 | Niccole Thurman als Mayor Flemming (voice) |
 | Sam Vincent als Krypto (voice) |
 | Brian Drummond als Streaky (voice) |
 | Scott McNeil als Ace (voice) |
| Cecilia Aranovich | Director |
| T.K. O'Brian | Writer |
| Rick Morales | Producer |
| James Krieg | Producer |
| Sam Register | Executive Producer |
| William Moulton Marston | Characters |
| Joe Shuster | Characters |
| Jerry Siegel | Characters |
| Bill Finger | Characters |
| Bob Kane | Characters |
| Jerry Robinson | Characters |
| Paul Dini | Characters |
| Bruce Timm | Characters |
| Joe Ruby | Thanks |
| Ken Spears | Thanks |
| Wes Gleason | Casting |
| Kyle Stafford | Editor |
| Robert J. Kral | Original Music Composer |
| Dawn Merkel | Line Producer |
| Christopher G. Collins | Production Manager |
| Ashley Malaski | Production Manager |
| Jason Amodeo | Assistant Production Manager |
| Daniel Nix | Assistant Production Manager |
| Steven Choi | Character Designer |
| Shane Glines | Character Designer |
| Andy Chiang | Prop Designer |
| Rodel Gravo | Background Designer |
| Jonard Soriano | Background Designer |
| Bill Dunn | Other |
| Mike Inman | Other |
| Timothy Szabo | Other |
| Richard Ziehler-Martin | Other |
| Nora Aillinn Murphy Berden | Color Designer |
| Cheng-Li Chan | Color Designer |
| Mira Lark Crowell | Color Designer |
| Maribel Pozos | Color Designer |
| Aluir Amancio | Storyboard Artist |
| Richard Gaines | Storyboard Artist |
| Miyuki Hoshikawa | Storyboard Artist |
| Brandon McKinney | Storyboard Artist |
| Joonki Park | Storyboard Artist |
| Adam Van Wyk | Storyboard Artist |
| Annie De Brock | Other |
| Anthony Akira Andrade | Animation |
| Elizabeth McMahill | Animation |
| Jason Plapp | Animation |
| Matthew Girardi | Art Direction |
| Brenna Kraus | Graphic Designer |
| Brenna Kraus | Title Designer |
| Jinhyun Choi | Animation Director |
| Scarlet Sookyung Kim | Animation Director |
| Kyoung-Soo Lee | Animation Director |
| Gyungsang Yoo | Animation Director |
| Kabcheon Jung | Layout |
| Dongkyu Lee | Layout |
| Dong-Hwan Oh | Layout |
| Sang Il Sim | Layout |
| Jang Sung-ho | Key Animation |
| Yonkoo Kang | Key Animation |
| Nam-Gil Jo | Key Animation |
| Yong Ahn | Painter |
| Bumji Kim | Painter |
| Woorye Jung | Painter |
| Eddie Jaeil Byun | CG Artist |
| Daeguen Hong | CG Artist |
| Hongsun Yoon | CG Artist |
| Sungtae Oh | Other |
| Steven Sh Yoon | Other |
| Agatha Sarim Kim | Other |
| Sujin Jung | Other |
| Ashley Hee-Eun Bae | Other |
| Greg Emerson | Other |
| Kip Brown | Post Producer |
| Rebecca Sands Coutts | Post-Production Manager |
| Marissa Llano | Post-Production Manager |
| Lisa Hallbauer | Producer |
| Sharon Yvonne Lopez | Production Manager |
| Nick Bannon | Assistant Production Manager |
| Marina Mozée | Assistant Production Manager |
| Jeff O. Collins | Other |
| Wilson Martinez | Other |
| Fred Salinas | Other |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| David M. Cowan | Dialogue Editor |
| David M. Cowan | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| John F. Reynolds | Dialogue Editor |
| John F. Reynolds | ADR Editor |
| Harrison T. Barth | Assistant Editor |
| Michele Beeson | Assistant Editor |
| Gabriel Gianola | Assistant Editor |
| Aisha Clarke | Administration |
| Tamara Miles | Other |
| Niko Sumcad | Other |
| Maria Millage | Other |
| Shelly Jacobs | Other |
| Cher Bentley | Other |
| Kira Tirimacco | Other |
| Renee Toporzysek | Other |
| Agnes Kim | Casting |
| Sarah Noonan | Casting |
| Liz Carroll | Casting |
| Yadira Davis | Production Accountant |
| Maral Simonian | Production Accountant |
| Athena Wingate | Production Accountant |
| Jake Baumgart | Other |
| Leslie Chinchilla | Legal Services |
| Raymond Gonzales | Legal Services |
| Shannon Muir | Legal Services |
| Iman Wilson | Legal Services |
| Bobbie Page | Production Supervisor |
| Liz Marshall | Production Supervisor |
| Ed Adams | Production Manager |
| Jay Bastian | Executive In Charge Of Production |
