 | Frank Welker als Scooby-Doo / Fred Jones (voice) |
 | Mindy Cohn als Velma Dinkley (voice) |
 | Grey DeLisle als Daphne Blake (voice) |
 | Matthew Lillard als Shaggy Rogers (voice) |
 | Obba Babatundé als Vampire Actor #4 (voice) |
 | Jeff Bennett als Vincent Van Helsing / Valdronya (voice) |
 | Julianne Buescher als Vampire Actor #3 / Reporter (voice) |
 | Christian Campbell als Bram (voice) |
 | Jim Cummings als Jesper Poubelle / Tulie (voice) |
 | Rob Paulsen als Sheriff / Vampire Actor #2 / Teen Vampire (voice) |
 | Mindy Sterling als Lita Rutland (voice) |
 | Robert Townsend als Vampire Actor #1 (voice) |
 | Jim Wise als Henry (voice) |
| David Block | Director |
| Tom Sheppard | Writer |
| Andrea Romano | Casting |
| Eric Semones | Art Direction |
| Matthew Girardi | Visual Effects |
| David Vasquez | Production Manager |
| Jay Bastian | Production Manager |
| Jeff Prezenkowski | Production Manager |
| Spike Brandt | Producer |
| Tony Cervone | Producer |
| Andy Sturmer | Original Music Composer |
| Kyle Stafford | Editor |
| Ted Blackman | Art Direction |
| Alan Burnett | Co-Producer |
| A.J. Vargas | Associate Producer |
| Sam Register | Executive Producer |
| Scott Shinick | Post-Production Manager |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| Christopher D. Lozinski | Online Editor |
| Steven White | Online Editor |
| Damon Criswell | Additional Music |
| Niki Sherrod | Other |
| Liz Carroll | Administration |
| Bobbie Page | Production Supervisor |
| Ed Adams | Production Manager |
| Jay Bastian | Executive In Charge Of Production |
| Jeff Prezenkowski | Executive In Charge Of Production |
| Joe Ruby | Thanks |
| Ken Spears | Thanks |
