 | Frank Welker als Scooby-Doo / Fred Jones (voice) |
 | Casey Kasem als Shaggy Rogers (voice) |
 | Mindy Cohn als Velma Dinkley (voice) |
 | Grey DeLisle als Daphne Blake (voice) |
 | Ron Perlman als Captain Skunkbeard / Biff Wellington (voice) |
 | Freddy Rodríguez als Rupert Garcia (voice) |
 | Tim Conway als Skip Jones (voice) |
 | Edie McClurg als Peggy Jones / Sea Salt Sally (voice) |
 | Kathy Najimy als Sunny St. Cloud (voice) |
 | Arsenio Hall als Captain Crothers (voice) |
 | Dan Castellaneta als Mr. Mysterio / Woodenleg Wally (voice) |
| Chuck Sheetz | Director |
| Margaret M. Dean | Writer |
| Jed Elinoff | Writer |
| Scott Alan Thomas | Writer |
| Sun Jae Lee | Animation |
| Susan Edmunson | Editor |
| Steven Argila | Original Music Composer |
| Margaret M. Dean | Producer |
| Chuck Sheetz | Producer |
| Kelly Ward | Casting |
| Joseph Barbera | Executive Producer |
| Sander Schwartz | Executive Producer |
| Christopher Keenan | Other |
| Vera Hourani | Associate Producer |
| Kelly Ward | Casting |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Michael Garcia | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Devon Bowman | Sound Mixer |
| Jeff Kettle | Other |
| Paul Menichini | Sound Designer |
| Paul Menichini | Sound Effects Editor |
| Sanaa Kelley | Foley Artist |
| Jeff Kettle | Foley Mixer |
| Tom Maydeck | Sound Re-Recording Mixer |
| Mike Draghi | Sound Re-Recording Mixer |
| Steven Argila | Orchestrator |
| Christopher D. Lozinski | Online Editor |
| Stephen Krause | Other |
| Liz Carroll | Administration |
| Andy Lewis | Production Manager |
