 | Frank Welker als Scooby-Doo / Rudy / Fred / Nefario (voice) |
 | Grey DeLisle als Daphne / Daisy / Musketeer 1 / Olive (voice) |
 | Matthew Lillard als Shaggy / Craggly / Captain Cutler (voice) |
 | Kate Micucci als Velma / Helga (voice) |
 | Myrna Velasco als Coco Diablo (voice) |
 | Dee Bradley Baker als Esteban / Mr. Wickles / Cat Man (voice) |
 | Jeff Bennett als Shady Guy / Charlie Humdrum / Hank (voice) |
 | Anthony Carrigan als Trevor Glume / Farmer (voice) |
 | Erin Fitzgerald als Library Kid / Superhero Girl / Musketeer 2 (voice) |
 | David Lodge als Warden Collins / Harry the Hypnotist / Inspector (voice) |
 | Lara Jill Miller als Prisoner Costume Teen / Superhero Boy / Musketeer 3 (voice) |
 | Candi Milo als Prison Woman / Dinosaur Kid / Monster Kid (voice) |
 | Jenelle Lynn Randall als Librarian / Superboy (voice) |
 | Kevin Michael Richardson als Sheriff / Henry Bascombe / 10,000 Volt Ghost (voice) |
| Sam Register | Executive Producer |
| Joe Goyette | Line Producer |
| Ryan Shore | Original Music Composer |
| John Soares | Editor |
| Sarah Noonan | Casting |
| Agnes Kim | Casting |
| Sara Jane Sherman | Other |
| Laura Pollak | Teleplay |
| Daniel McLellan | Teleplay |
| Daniel McLellan | Story |
| Robert Ehrenreich | Editor |
| Audie Harrison | Director |
| Audie Harrison | Executive Producer |
| Audie Harrison | Story |
| Audie Harrison | Teleplay |
| Yelim Heo | Production Manager |
| Alvaro Zelaya | Production Manager |
| Scotty Morrison | Assistant Production Manager |
| Cole Crawford Rothacker | Assistant Production Manager |
| Erik D. Martin | Art Direction |
| Mary Hoffman | Lead Character Designer |
| Thaddeus Couldron | Character Designer |
| Danny Kimanyen | Character Designer |
| Kirk Millett | Character Designer |
| Dave Sherburne | Character Designer |
| Kyle Bowman | Prop Designer |
| Sophia Chen | Prop Designer |
| Thaddeus Couldron | Prop Designer |
| Demetrice Wilson | Prop Designer |
| Lauren Duda | Background Designer |
| Victor Ha | Background Designer |
| Steven Hai | Background Designer |
| Jacques Lee | Background Designer |
| Richard Lee | Background Designer |
| Corey Loving | Background Designer |
| Kimberly Bitsui | Color Designer |
| David S. Karoll | Color Designer |
| Nancy Ulene | Color Designer |
| Andrew Bendik | Storyboard Artist |
| Ben Fosselman | Storyboard Artist |
| Aaron Fryer | Storyboard Artist |
| Carolyn Gair | Storyboard Artist |
| Sean Glaze | Storyboard Artist |
| Ye Aung | Animation |
| Will Cuhn | Animation |
| Dan Haskett | Animation |
| Jeffrey J. Zikry | Animation |
| Brenna Kraus | Graphic Designer |
| Brenna Kraus | Title Designer |
| Adrienne Steinbaum | Graphic Designer |
| Adrienne Steinbaum | Title Designer |
| Kaine Patel | Production Supervisor |
| Jonathon Tinsay | Production Supervisor |
| Romy Garcia | Production Supervisor |
| Dennis Tomacruz | Animation Director |
| Adrian Lasquite | Key Animation |
| Alexander Ferrarez | Key Animation |
| Angelo Teoxon | Key Animation |
| Raquel Cutright | Layout Supervisor |
| Akiblas Flores | Special Effects Supervisor |
| Arvin Villarin | Special Effects |
| Calvin Macapallag | Special Effects |
| Nelson Sambo | Effects Supervisor |
| Erick Austria | Compositing Supervisor |
| Anna Theresa Tabalba | Painter |
| Baltazar Masucol | Painter |
| Charles Derith | Painter |
| Joel De Leon | Production Manager |
| Emmanuel Palad | Technical Supervisor |
| Khristian Domantay | Technical Supervisor |
| Nathaniel Trinidad | Technical Supervisor |
| Greg Emerson | Other |
| Kip Brown | Post Producer |
| Rebecca Sands Coutts | Post-Production Manager |
| Marissa Llano | Post-Production Manager |
| Lisa Hallbauer | Other |
| Emily Dillard | Assistant Production Manager |
| Mark A. Keatts | Supervising Dialogue Editor |
| David M. Cowan | Dialogue Editor |
| David M. Cowan | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| John F. Reynolds | Dialogue Editor |
| John F. Reynolds | ADR Editor |
| Fred Salinas | Dialogue Editor |
| Darleen Stoker | ADR Editor |
| David Cowles | Online Editor |
| Harrison T. Barth | Assistant Editor |
| Michele Beeson | Assistant Editor |
| Gabriel Gianola | Assistant Editor |
| Vinicius Barbosa Pippa | Music Coordinator |
| David Bawiec | Additional Music |
| Fred Emory Smith | Additional Music |
| Liz Carroll | Administration |
| Maria Millage | Casting |
| Chaz Murphy | Production Accountant |
| Athena Wingate | Production Accountant |
| Bobbie Page | Production Supervisor |
| Liz Marshall | Production Supervisor |
| Ed Adams | Production Manager |
| Sammy Perlmutter | Executive In Charge Of Production |
| Joe Ruby | Thanks |
| Ken Spears | Thanks |
| Joseph McCormick | Background Designer |
| Nath Milburn | Background Designer |
| Reid Monninger | Background Designer |
| Gary Montalbano | Background Designer |
| Trevor Spencer | Background Designer |
| Ernie Keen | Storyboard Artist |
| Dylan King | Storyboard Artist |
| Mike Milo | Storyboard Artist |
| Jackson Read | Storyboard Artist |
| Aaron Springer | Storyboard Artist |
| Mark A. Keatts | Supervising ADR Editor |
| Ginger Brown | Associate Editor |
| Charlie Carpenter | Assistant Production Manager |
