 | Frankie Muniz als Stripes (voice) |
 | Hayden Panettiere als Channing Walsh |
 | Bruce Greenwood als Nolan Walsh |
 | Mandy Moore als Sandy (voice) |
 | Wendie Malick als Clara Dalrymple |
 | M. Emmet Walsh als Woodzie |
 | Snoop Dogg als Lightning (voice) |
 | Michael Clarke Duncan als Clydesdale (voice) |
 | Jeff Foxworthy als Reggie (voice) |
 | Whoopi Goldberg als Franny (voice) |
 | Steve Harvey als Buzz (voice) |
 | Dustin Hoffman als Tucker (voice) |
 | Joshua Jackson als Trenton's Pride (voice) |
 | Joe Pantoliano als Goose (voice) |
 | Michael Rosenbaum als Ruffshodd (voice) |
 | David Spade als Scuzz (voice) |
 | Gary Bullock als John Cooper |
 | Fred Thompson als Sir Trenton (voice) |
 | Caspar Poyck als Mailman |
| Thandi Puren als Reporter |
 | Morné Visser als Reporter |
| Dawn Matthews als Reporter |
 | Matt Stern als Reporter |
| John Lesley als Paddock Boss |
| Graeme Hawkins als Track Announcer |
| Tarryn Meaker als Anthem Singer |
 | Jansen Panettiere als Young Stripes (voice) |
 | Frankie Ryan Manriquez als Young Ruffshodd (voice) |
| Kyle Alcazar als Young Pride (voice) |
 | Dee Bradley Baker als Additional Voices (voice) |
 | Bob Bergen als Additional Voices (voice) |
 | Jess Harnell als Additional Voices (voice) |
| Mark Isham | Original Music Composer |
| Broderick Johnson | Producer |
| David Eggby | Director of Photography |
| David Schmidt | Screenplay |
| Frederik Du Chau | Director |
| Tom Finan | Editor |
| Andrew A. Kosove | Producer |
| Edward McDonnell | Producer |
| Lloyd Phillips | Producer |
| Steven P. Wegner | Executive Producer |
| David Schmidt | Story |
| Steven P. Wegner | Story |
| Kirk DeMicco | Story |
| Frederik Du Chau | Story |
| Philip A. Patterson | Co-Producer |
| Kira Davis | Co-Producer |
| Kirk DeMicco | Co-Producer |
| Wolf Kroeger | Production Design |
| Deva Anderson | Music Supervisor |
| Amanda Mackey | Casting Director |
| Cathy Sandrich Gelfond | Casting Director |
| Lloyd Phillips | Unit Production Manager |
| Philip A. Patterson | First Assistant Director |
| Jamie Marshall | Second Assistant Director |
| Genevieve Hofmeyr | Line Producer |
| John Mahaffie | Second Unit Director |
| Angela Phillips | Production Manager |
| Jan S. Owen | Post Production Supervisor |
| Brad Arensman | Post Production Supervisor |
| Julie Huntsinger | Associate Producer |
| Kent Houston | Visual Effects Supervisor |
| Dion Hatch | Visual Effects Supervisor |
| Eric Mises-Rosenfeld | Visual Effects Supervisor |
| Mary Stuart | Visual Effects Producer |
| Rod Park | Visual Effects Producer |
| Alex Williams | Animation Supervisor |
| Guy Micheletti | Key Grip |
| Jonathan Hely-Hutchinson | Art Direction |
| Emilia Roux | Set Decoration |
| Karl Lewis Miller | Animal Coordinator |
| Jo Katsaras | Costume Design |
| Neil McClean | Costume Supervisor |
| Nadine Prigge | Key Makeup Artist |
| Jacques Gerber | Key Hair Stylist |
| Yolanda T. Cochran | Production Controller |
| Deon Du Preez | Location Manager |
| Cordell McQueen | Special Effects Supervisor |
| Matthew Merkovich | CGI Supervisor |
| Fortunato Frattasio | Digital Effects Supervisor |
| Brennan Prevatt | Digital Effects Supervisor |
| Randy Dormans | Lead Animator |
| Lawrence Carroll | Compositing Lead |
| Rob Ozaeta | Compositing Lead |
| Antoinette Perez | Visual Effects Coordinator |
| Keith Luczywo | Visual Effects Coordinator |
| Terron Pratt | Visual Effects Coordinator |
| Thierry Delattre | Visual Effects Supervisor |
| Richard Martin | Visual Effects Supervisor |
| Pierre Couture | Visual Effects Supervisor |
| Paul Docherty | Digital Effects Supervisor |
| Diane Kingston | Digital Effects Producer |
| George Port | Visual Effects Supervisor |
| Carol Petrie | Visual Effects Coordinator |
| Kim Richardson | Lead Animator |
| Curtis Roush | Supervising Music Editor |
