 | Megan Fox als April O'Neil |
 | Will Arnett als Vern Fenwick |
 | William Fichtner als Eric Sacks |
 | Alan Ritchson als Raphael |
 | Noel Fisher als Michelangelo |
 | Pete Ploszek als Leonardo |
 | Johnny Knoxville als Leonardo (voice) |
 | Jeremy Howard als Donatello |
 | Danny Woodburn als Splinter |
 | Tony Shalhoub als Splinter (voice) |
 | Tohoru Masamune als Shredder |
 | Whoopi Goldberg als Bernadette Thompson |
 | Minae Noji als Karai |
 | Abby Elliott als Taylor |
 | Madison Mason als Councilman |
 | Taran Killam als McNaughton |
 | K. Todd Freeman als Dr. Baxter Stockman |
 | Paul Fitzgerald als Dr. O'Neil |
 | Malina Weissman als Young April O'Neil |
 | Venida Evans als Subway Hostage |
 | Mikal Vega als Sacks Bodyguard |
 | Harley Pasternak als Harley Pasternak |
| Braeson Herold als Reporter |
 | Chris Wylde als Reporter |
 | Chance Kelly als Mr. Rivetti |
 | Rick Chambers als News Anchor |
| Leyna Nguyen als News Anchor |
 | Derek Mears als Dojo Ninja |
| Bradley Fuller | Producer |
| Michael Bay | Producer |
| Andrew Form | Producer |
| Eric J. Crown | Executive Producer |
| Galen Walker | Producer |
| Scott Mednick | Producer |
| Peter Laird | Characters |
| Evan Daugherty | Writer |
| Josh Appelbaum | Writer |
| André Nemec | Writer |
| Kevin Eastman | Characters |
| Debra Zane | Casting |
| Kathleen Chopin | Casting |
| Ian Bryce | Producer |
| Denis L. Stewart | Executive Producer |
| Napoleon Smith III | Executive Producer |
| Jason Reed | Executive Producer |
| Lula Carvalho | Director of Photography |
| Neil Spisak | Production Design |
| Joel Negron | Editor |
| Glen Scantlebury | Editor |
| Sarah Edwards | Costume Designer |
| Brian Tyler | Original Music Composer |
| Pablo Helman | Visual Effects Supervisor |
| Basil Grillo | Unit Production Manager |
| Denis L. Stewart | Unit Production Manager |
| Lyn Pinezich | Unit Production Manager |
| Darin Rivetti | First Assistant Director |
| Maggie Murphy | Second Assistant Director |
| Susan MacLeod | Visual Effects Producer |
| Tim Harrington | Animation Supervisor |
| Susan Greenhow | Visual Effects Producer |
| Ethan van der Ryn | Supervising Sound Editor |
| Erik Aadahl | Supervising Sound Editor |
| Laurent Kossayan | Supervising Sound Editor |
| Terry Porter | Sound Re-Recording Mixer |
| Anna Behlmer | Sound Re-Recording Mixer |
| David Leitch | Stunt Coordinator |
| Jonathan Eusebio | Stunt Coordinator |
| Garrett Warren | Stunt Coordinator |
| Hank Amos | Stunt Coordinator |
| Luciano Acuna Jr. | Stunts |
| Christopher 'Critter' Antonucci | Stunts |
| Airon Armstrong | Stunts |
| Bobby Beckles | Stunts |
| Brandon Beckman | Stunts |
| Randy James Beckman | Stunts |
| John Bernecker | Stunts |
| Daniel Bernhardt | Stunts |
| Nitasha Bhambree | Stunts |
| Chad Bowman | Stunts |
| Nikki Brower | Stunts |
| Scott Burik | Stunts |
| Bryce Burke | Stunts |
| Jared Burke | Stunts |
| Gabriella Chabrier | Stunts |
| Anis Cheurfa | Stunts |
| Jacob Dewitt | Stunts |
| Rockey Dickey Jr. | Stunts |
| Jared S. Eddo | Stunts |
| Matt Emig | Stunts |
| Peter Epstein | Stunts |
| Kyle Gardiner | Stunts |
| Jeffrey Lee Gibson | Stunts |
| Daniel Graham | Stunts |
| Daniel Hernandez | Stunts |
| Chad Hessler | Stunts |
| Cort Hessler | Stunts |
| Harry Humphries | Stunts |
| Stephen Izzi | Stunts |
| Aaron Joshua | Stunts |
| Adam Kirley | Stunts |
| Pete Klein | Stunts |
| Suo Liu | Stunts |
| Samantha MacIvor | Stunts |
| Alexa Marcigliano | Stunts |
| Jeremy Marinas | Stunts |
| Michael McGuire | Stunts |
| Declan Mulvey | Stunts |
| Jason Ng | Stunts |
| Jim Ng | Stunts |
| Christopher Place | Stunts |
| Thomas Place | Stunts |
| Tracey Ruggiero | Stunts |
| Cale Steven Schultz | Stunts |
| Hannah Scott | Stunts |
| Philip J. Silvera | Stunts |
| Buddy Sosthand | Stunts |
| Jackson Spidell | Stunts |
| Kimmy Suzuki | Stunts |
| Don Theerathada | Stunts |
| Luis Tolentino | Stunts |
| Jon Valera | Stunts |
| Aaron Vexler | Stunts |
| Anthony Vincent | Stunts |
| Joe Nin Williams | Stunts |
| Adam Wood | Stunts |
| Basil Grillo | Associate Producer |
| Marc Pappalardo | Associate Producer |
| Bret Rowe | Associate Producer |
| Lori Scowley | Associate Producer |
| Lori Scowley | Production Controller |
| Debra Schutt | Set Decoration |
| Lisa Scoppa | Set Decoration |
| David Emmerichs | Steadicam Operator |
| David Emmerichs | "A" Camera Operator |
| Tim Norman | "B" Camera Operator |
| Bobby Mancuso | First Assistant Camera |
| Chris Silano | First Assistant Camera |
| Scott Tinsley | Second Assistant Camera |
| Anthony Coan | Second Assistant Camera |
| Meg Kettell | Second Assistant Camera |
| Justin Mancuso | Camera Loader |
| Nicholas Kay | Digital Imaging Technician |
| Shane B. Scott | Script Supervisor |
| William Sarokin | Sound Mixer |
| George Leong | Boom Operator |
| Schavaria Reeves | Utility Sound |
| Jay Fortune | Chief Lighting Technician |
| Brian McClean | Assistant Chief Lighting Technician |
| Diana Burton | Property Master |
| Yolan Fisher | Assistant Property Master |
| Burt Dalton | Special Effects Supervisor |
| Terry Chapman | Special Effects Technician |
| Joseph Martin | Special Effects Technician |
| Dimitra Bixby | Special Effects Technician |
| Aleksandr Lokensgard | Special Effects Technician |
| Christopher Jones | Special Effects Technician |
| Michael Roundy | Special Effects Technician |
| James Henry | Special Effects Technician |
| Jose Antonio Paramo | Special Effects Technician |
| Rick Figalan | Special Effects Technician |
| Eric Jolley | Special Effects Technician |
| Ryan Roundy | Special Effects Technician |
| John Greene | Special Effects Technician |
| Frazer Newton | Special Effects Technician |
| Bruce Y. Kuroyama | Special Effects Technician |
| Eugene Hitt | Special Effects Technician |
| Christy Cham | Assistant Costume Designer |
| Emily Gunshor | Assistant Costume Designer |
| Katie Riley | Assistant Costume Designer |
| Kate Edwards | Costume Supervisor |
| Megan Asbee | Costume Supervisor |
| Kimmie Guiragossian | Costumer |
| Sabrina Calley | Costumer |
| Courtney D'Alesio | Costumer |
| Pam Aaron | Costumer |
| Mindy Hall | Makeup Department Head |
| Christine Domaniecki | Makeup Artist |
| Ashley Ryan | Makeup Artist |
| Bridget Ritzinger | Makeup Artist |
| Mary McNamara | Makeup Artist |
| Mandy Lyons | Hair Department Head |
| Christine Fennell | Key Hair Stylist |
| April Schuller | Hairstylist |
| Rose Chatterton | Hairstylist |
| Scott Sullens | Production Coordinator |
| Lauren Butler | Assistant Production Coordinator |
| Veronique Lee | Assistant Production Coordinator |
| David E. Bisson | Assistant Production Coordinator |
| Sean Murray | Assistant Production Coordinator |
| Jeremy Marks | Second Second Assistant Director |
| Cathleen McFadyen | Additional Second Assistant Director |
| Maria T. Bierniak | Location Manager |
| Justin Rosini | Assistant Location Manager |
| Tannis Vallely | Casting Assistant |
| Shayna Markowitz | Casting Assistant |
| Rori Bergman | Casting Associate |
| Meghan Hart | Casting Assistant |
| Elijah Cornell | Extras Casting Assistant |
| Tristan Paris Bourne | Assistant Art Director |
| Peter Dorsey | Assistant Art Director |
| Ruth Falco | Assistant Art Director |
| Jeffrey D. McDonald | Assistant Art Director |
| Holly Watson | Graphic Designer |
| Todd Harris | Storyboard Artist |
| Michael Anthony Jackson | Storyboard Artist |
| Mark Vena | Storyboard Artist |
| Rebecca Meis DeMarco | Set Decoration Buyer |
| Philip Canfield | Leadman |
| Kelly Solomon | Set Decorating Coordinator |
| Deborah Canfield | Set Dresser |
| Teresa Kelly Canfield | Set Dresser |
| Nick Miller | Construction Coordinator |
| Susan E. Novick | Post Production Supervisor |
| Peter Gvozdas | Additional Editing |
| Jim May | Additional Editing |
| Tommy Aagaard | First Assistant Editor |
| Kevin Stermer | Assistant Editor |
| Donna Madrigal | Assistant Editor |
| Holly Isaacson | Post Production Assistant |
| Natalie Setoute | Post Production Assistant |
| Jeremy Bradley | Visual Effects Editor |
| Rebecca Weigold | Visual Effects Editor |
| Shelly Lloyd-Samson | Visual Effects Production Manager |
| Kraig Tytus | Visual Effects Coordinator |
| Meghan L. Noble | 3D Editor |
| Zahida Bacchus | 3D Supervisor |
| Kayla Franklin | 3D Coordinator |
| Polly McKinnon | ADR Supervisor |
| Jason W. Jennings | Sound Designer |
| Lee Gilmore | Sound Effects Editor |
| Phil Barrie | Sound Effects Editor |
| Bob Kellough | Sound Effects Editor |
| Jonathan Klein | Foley Supervisor |
| Scott Curtis | Foley Editor |
| David V. Butler | Dialogue Editor |
| Susan Dudeck | ADR Editor |
| Jesse Rosenman | Assistant Sound Editor |
| Dan O'Connell | Foley Artist |
| John T. Cucci | Foley Artist |
| Alicia Stevenson | Foley Artist |
| Dawn Fintor | Foley Artist |
| James Ashwill | Foley Mixer |
| John Guentner | Foley Mixer |
| David Jobe | Foley Mixer |
| Barbara Harris | ADR Voice Casting |
| Michael Miller | ADR Mixer |
| Tim West | ADR Mixer |
| Judah Getz | ADR Mixer |
| Frank Verderosa | ADR Mixer |
| Chris Navarro | ADR Mixer |
| Joe Lisanti | Music Editor |
| Mitchell Amundsen | Second Unit Director of Photography |
| Chris Castaldi | Second Unit First Assistant Director |
| David Fischer | Second Assistant Director |
| Julian J. Delacruz | First Assistant Camera |
| Alex Worster | Second Assistant Camera |
| Keith Putnam | Digital Imaging Technician |
| J.D. Schwalm | Special Effects Supervisor |
| Stuart Nicolai | Location Manager |
| Jill Alexander | Property Master |
| Scott Rogers | Stunt Coordinator |
| Stanton Barrett | Stunts |
| Tad Griffith | Stunts |
| Ryan Hanna | Stunts |
| Gwyneth E. Larsen | Stunts |
| Oakley Lehman | Stunts |
| Sam Ly | Stunts |
| Michael Erik Majesky | Stunts |
| Hiroo Minami | Stunts |
| Jim Wilkey | Stunts |
| Danny Wynands | Stunts |
| Pablo Helman | Second Unit Director |
| Mark Trapenberg | Second Unit First Assistant Director |
| Thomas K. Lee | Second Assistant Director |
| Patrick Capone | Second Unit Director of Photography |
| David B. Nowell | Aerial Director of Photography |
| Jonathan Beck | "A" Camera Operator |
| David M. Dunlap | "B" Camera Operator |
| Gerard Sava | "B" Camera Operator |
| Gerard Sava | Steadicam Operator |
| Braden Belmonte | First Assistant Camera |
| Ben Dailey | First Assistant Camera |
| John Oliveri | Second Assistant Camera |
| Caitlin Machak | Second Assistant Camera |
| Robert Strait | Digital Imaging Technician |
| Katri Billard | Script Supervisor |
| Linda Haftel | Script Supervisor |
| Mathew Price | Sound Mixer |
| William O'Leary | Chief Lighting Technician |
| Elizabeth Schlitten | Property Master |
| Rachael Weinzimer | Property Master |
| Raymond L. Negron | Second Second Assistant Director |
| Tom Glick | Second Second Assistant Director |
| Adam Bernard | Additional Second Assistant Director |
| Allegra Clegg | Unit Production Manager |
| Daren Hicks | Production Supervisor |
| Mark Cotone | Second Unit First Assistant Director |
| Eric Glasser | Second Assistant Director |
| Jon Recher | Second Second Assistant Director |
| Amanda Moss Serino | Set Decoration |
| Duane Manwiller | Camera Operator |
| Coy Aune | Camera Operator |
| Jimmy E. Jensen | First Assistant Camera |
| E.J. Misisco | First Assistant Camera |
| Brent Egan | Second Assistant Camera |
| Jeff Tomcho | Digital Imaging Technician |
| Jonathan Hook | Location Manager |
| Michael Wesley | Assistant Location Manager |
| Kris Smith | Script Supervisor |
| Walter Anderson | Sound Mixer |
| Glenn Young | Boom Operator |
| Bob E. Krattiger | Chief Lighting Technician |
| Thomas M. Dangcil | Assistant Chief Lighting Technician |
| David Amborn | Special Effects Coordinator |
| Eric Frazier | Special Effects Coordinator |
| Chris Burton | Special Effects Coordinator |
| Todd Ellis | Property Master |
| Allison Gross | Assistant Property Master |
| Bart Barbuscia | Leadman |
| Angelo Bua | Set Dresser |
| Genelle Ciccarelli | Set Dresser |
| Geoffrey Nightingale | Set Dresser |
| Lisa Lovaas | Costume Supervisor |
| Chrissy Callan | Assistant Costume Designer |
| Sabrina Calley | Set Costumer |
| Jaclyn Tamizato | Set Costumer |
| Christie Kwan | Production Coordinator |
| Brianna Hoskins | Assistant Production Coordinator |
| Masako Masuda | Set Designer |
| Monica Fedrick | Graphic Designer |
| Robert Consing | Storyboard Artist |
| Eddie Campbell | Set Decorating Coordinator |
| Charlotte Parker | Hair Department Head |
| Fríða Aradóttir | Hairstylist |
| Denise Paulson | Makeup Department Head |
| Michelle Vitone | Makeup Artist |
| Maryellen Aviano | Extras Casting |
| Dave DeGaetano | Construction Coordinator |
| Kristina Baskett | Stunts |
| Chris Brewster | Stunts |
| Gui DaSilva-Greene | Stunts |
| Jef Groff | Stunts |
| Jake Huang | Stunts |
| Brendon Huor | Stunts |
| Nito Larioza | Stunts |
| Kurt D. Lott | Stunts |
| Kyle Mclean | Stunts |
| Heather Elwell | Art Department Coordinator |
| Madeline Austin-Kulat | Art Department Coordinator |
| Miguel López-Castillo | Art Direction |
| Patricio M. Farrell | Art Direction |
| Alberto Gonzalez-Reyna | Assistant Art Director |
| Blythe R.D. Quinlan | Assistant Art Director |
| Victoria Ruskin | Assistant Art Director |
| Dan Bradley | Second Unit Director |
| David Leitch | Second Unit Director |
| Jonathan Liebesman | Director |
