 | Micah Abbey als Donatello (voice) |
 | Shamon Brown Jr. als Michelangelo (voice) |
 | Nicolas Cantu als Leonardo (voice) |
 | Brady Noon als Raphael (voice) |
 | Ayo Edebiri als April O'Neil (voice) |
 | Maya Rudolph als Cynthia Utrom (voice) |
 | John Cena als Rocksteady (voice) |
 | Seth Rogen als Bebop (voice) |
 | Rose Byrne als Leatherhead (voice) |
 | Natasia Demetriou als Wingnut (voice) |
 | Giancarlo Esposito als Baxter Stockman (voice) |
 | Jackie Chan als Splinter (voice) |
 | Ice Cube als Superfly (voice) |
 | Paul Rudd als Mondo Gecko (voice) |
 | Post Malone als Ray Fillet (voice) |
 | Hannibal Buress als Genghis Frog (voice) |
 | Jimmy Donaldson als Times Square Guy (voice) |
 | Derek Wilson als Spider (voice) |
 | Lukas Williams als Curious Goon (voice) |
 | Michael Badalucco als Bad Bernie (voice) |
| Dempsey Pappion als Bald Bronson (voice) |
 | John Capodice als Cab Driver (voice) |
| Andia Winslow als TV Anchor (voice) |
 | Raechel Wong als News Anchor (voice) |
 | David Faustino als Normal Nate (voice) |
 | Danny Mastrogiorgio als Toupee Tom / Police Officer (voice) |
| Noel Gibson als Responding Police Officer / Military Advisor (voice) |
| Myra Owyang als Short Sharon (voice) |
 | Kevin Eastman als Good Human (voice) |
 | Alex Hirsch als Bossy Goon / Scumbug (voice) |
 | Mike Rianda als Paycheck Goon / Chop Shop Boss / Ratatouille Guard (voice) |
| Bobby Wagner als Man Who Thought a Giant Rat was a Big Cat but was a Rat (voice) |
 | Greg Levitan als Gecko Goon (voice) |
| Illya Quinteros als Helpful Construction Worker (voice) |
 | Natalie Canizares als Sign My Baby Mom (voice) |
 | Kyler Spears als Apologetic Goon (voice) |
 | Jeff Rowe als Man Who Loves Being Young and Free to Go Places (voice) |
| Jeff Rowe | Director |
| Evan Goldberg | Producer |
| Seth Rogen | Producer |
| James Weaver | Producer |
| Kyler Spears | Co-Director |
| Yebin Kang | Visual Development |
| Adel Sabi | Character Designer |
| Daniela Kriston | Researcher |
| Kellan Jett | Visual Development |
| Kevin Eastman | Characters |
| Peter Laird | Characters |
| Trent Reznor | Original Music Composer |
| Atticus Ross | Original Music Composer |
| Garrett Lee | Visual Development |
| Brendan O'Brien | Story |
| Seth Rogen | Story |
| Evan Goldberg | Story |
| Jeff Rowe | Story |
| Gabe Hilfer | Music Supervisor |
| Lukas Williams | Co-Producer |
| Ramsay McBean | Executive Producer |
| Josh Fagen | Executive Producer |
| Seth Rogen | Screenplay |
| Evan Goldberg | Screenplay |
| Jeff Rowe | Screenplay |
| Benji Samit | Screenplay |
| Dan Hernandez | Screenplay |
| Chris Kazmier | Visual Effects Supervisor |
| Andrew Joustra | Script Coordinator |
| Cindy Y. Avila | Production Coordinator |
| Woodrow White | Lead Character Designer |
| James A. Castillo | Character Designer |
| Maxime Mary | Character Designer |
| Justin Runfola | Character Designer |
| Jules Itzkoff | Visual Development |
| Eric Kissack | Additional Editing |
| Marie Balland | Co-Producer |
| Greg Levitan | Editor |
| Yashar Kassai | Production Design |
| Arthur Fong | Art Direction |
| Tiffany Lam | Art Direction |
| Gabriel Lin | Head of Story |
| Matthieu Rouxel | Visual Effects Supervisor |
| Jacques Daigle | Head of Animation |
| Simon Cuisinier | Head of Animation |
| Mark A. Mangini | Sound Designer |
| Rich Delia | Original Casting |
| Anna Jahn | Production Manager |
| Paige Caldwell | Story Artist |
| Sang Yup Lee | Story Artist |
| Isabella Spadone | Story Artist |
| Hanna Cho | Story Artist |
| Chloé Nicolay | Story Artist |
| Jacob Streilein | Story Artist |
| Peter Foltz | Story Artist |
| Charlie Parisi | Story Artist |
| Ray Xu | Story Artist |
| John Jackson | Story Artist |
| Andrew James Ross | Story Artist |
| Illya Quinteros | Associate Editor |
| Myra Owyang | Associate Editor |
| David Croomes | Associate Editor |
| Art Arreola | Assistant Editor |
| Eryn Suchara | Assistant Editor |
| David Bleich | Visual Development |
| Lauren Airriess | Visual Development |
| Nikita Chan | Visual Development |
| Dustin D'Arnault | Visual Development |
| Tom Eichacker | Visual Development |
| Nacho Molina | Visual Development |
| Lily Nishita | Visual Development |
| Sean Sevestre | Visual Development |
| Alger Tam | Visual Development |
| Jeffrey M. Thompson | Visual Development |
| Fred Wong | Visual Development |
| J.J. Villard | Visual Development |
| Natan Moura | Visual Development |
| Courtney Madincea | Production Supervisor |
| Derek Manzella | Production Supervisor |
| Yasmine Mzayek | Production Supervisor |
| Ketsia A. Védrine | Production Supervisor |
| Daniel Kahn | Production Coordinator |
| Ryan Kuo | Production Supervisor |
| Ryan Fragomeni | Production Coordinator |
| Leah Koerwer | Production Coordinator |
| Adam Richards | Casting Associate |
| Linda LaMontagne | Additional Casting |
| Adrianna A.J. Cohen | Executive Producer |
| Julien Meesters | Executive Producer |
| Maxence Verniers-Diers | Animation |
| Maxime Sirven | Assistant Editor |
| Nicolas Benoit | Lead Animator |
| Victor Hareng | Lead Animator |
| Martin Scalzotto | Lead Animator |
| David Carrière | Lead Animator |
| David Marquis | Lead Animator |
| Sarah Simon | Lead Animator |
| Tanguy Gourret | Lead Animator |
| Etienne Metois | Lead Animator |
| Jean-Paul Suau | Lead Animator |
| Florent Razafimandimby | Lead Animator |
| Chelsea Body | Foley Mixer |
| Christopher Bonis | Sound Effects Editor |
| Jennie Shea | ADR Recordist |
| Jesse Dodd | ADR Mixer |
| Jacob Flack | Sound Effects Editor |
| Kevin Jung | Foley Editor |
| Alex Gregson | Sound Re-Recording Mixer |
| Mark A. Mangini | Sound Re-Recording Mixer |
| Mark A. Mangini | Supervising Sound Editor |
| Michael Semanick | Sound Re-Recording Mixer |
| Scott Carroll | Head of Animation |
| Denis Tassenoy | Lighting Supervisor |
| Florent Masurel | 2D Artist |
| Anais Basso | 2D Artist |
| Basile Gouttenoire | 2D Artist |
| Romain Recher | Production Manager |
| Elisa Guibert | Production Coordinator |
| Tom Kurcz | Animation |
| Dana Gardner | Studio Teacher |
| Kent Seki | Other |
| Jorge Roldán Signes | Other |
