 | Jonathan Bennett als Bo Duke |
 | Randy Wayne als Luke Duke |
 | April Scott als Daisy Duke |
 | Joel David Moore als Cooter |
 | Harland Williams als Rosco P. Coltrane |
 | Sherilyn Fenn als Lulu Hogg |
 | Willie Nelson als Jesse Duke |
 | Christopher McDonald als JD "Boss" Hogg |
 | Todd Grinnell als Hughie Hogg |
 | Gary Cole als The Balladeer (voice) |
 | Jennifer Hill als Brooke Handy |
 | Trishelle Cannatella als Ali Handy |
 | Rufus Dorsey als DMV Examiner |
 | Joe Gieb als Carnival Midget |
 | Doug Jones als Patron |
 | Challen Cates als Psychic |
 | Alex Boling als Father |
 | Eve Sigall als Tough Woman |
 | Carrie Minter als Sunbathing Girl #1 |
 | Casey Durkin als Sunbathing Girl #2 |
 | Kym Stys als Mechanical Bullrider (uncredited) |
| Robert Berlinger | Director |
| Shane Morris | Writer |
| Gy Waldron | Writer |
| Brian Avery | Stunts |
| Roy H. Wagner | Director of Photography |
| Don Brochu | Editor |
| Robert Benjamin | Co-Producer |
| Bill Gerber | Producer |
| Phillip B. Goldfine | Producer |
| Taylor Latham | Associate Producer |
| Alison Semenza | Executive Producer |
| John DeFaria | Music |
| Robin Peyton | Set Decoration |
| Kandy Stern | Production Design |
| Kandy Stern | Art Direction |
| Peter Montagna | Makeup Artist |
| Tricia Gray | Costume Design |
| Melissa V. Barnes | Second Assistant Director |
| Maximillian D. Day | First Assistant Director |
| William H. Schirmer | Special Effects |
| Edward L. Williams | Visual Effects Supervisor |
| John F. Gross | Visual Effects Supervisor |
| Joe Bucaro III | Stunt Double |
| Jacob Chambers | Stunt Double |
| Bruce Paul Barbour | Stunt Driver |
| John Clagett | Stunt Driver |
| Michael Adams | Stunts |
| Courtney Farnsworth | Utility Stunts |
| Diamond Farnsworth | Stunt Coordinator |
| Chris LaCentra | Utility Stunts |
| Michael B. Johnson | Stunts |
| Tom McComas | Stunt Driver |
| Dina L. Margolin | Stunts |
| Tarah Paige | Stunts |
| Mike Mukatis | Stunt Double |
| Vanessa Motta | Stunts |
| Joni Podesta | Stunts |
| Rick Seaman | Stunts |
| Peewee Piemonte | Stunts |
| Kym Stys | Stunts |
| Brian J. Williams | Stunts |
| Brian Crane | Gaffer |
| Eric Anderson | Second Unit Director of Photography |
| Mark Lanza | Supervising Sound Editor |
| Melanie Mander | Script Supervisor |
