 | Chris Pratt als Mario (voice) |
 | Anya Taylor-Joy als Princess Peach (voice) |
 | Charlie Day als Luigi (voice) |
 | Jack Black als Bowser (voice) |
 | Keegan-Michael Key als Toad (voice) |
 | Seth Rogen als Donkey Kong (voice) |
 | Fred Armisen als Cranky Kong (voice) |
 | Sebastian Maniscalco als Spike (voice) |
 | Charles Martinet als Mario's Dad / Giuseppe (voice) |
 | Kevin Michael Richardson als Kamek (voice) |
 | Khary Payton als Penguin King (voice) |
 | Rino Romano als Uncle Tony (voice) |
 | John DiMaggio als Uncle Arthur (voice) |
 | Jessica DiCicco als Mario's Mom (voice) |
 | Eric Bauza als Toad General (voice) |
 | Juliet Jelenic als Lumalee (voice) |
 | Scott Menville als Koopa General (voice) |
 | Carlos Alazraqui als Additional Voices (voice) |
| Jason Broad als Additional Voices (voice) |
 | Ashly Burch als Additional Voices (voice) |
 | Rachel Butera als Additional Voices (voice) |
 | Cathy Cavadini als Additional Voices (voice) |
 | Will Collyer als Additional Voices (voice) |
| Django Craig als Additional Voices (voice) |
 | Willow Geer als Additional Voices (voice) |
 | Aaron Hendry als Additional Voices (voice) |
 | Andy Hirsch als Additional Voices (voice) |
 | Barbara Harris als Additional Voices (voice) |
 | Phil LaMarr als Additional Voices (voice) |
 | Jeremy Maxwell als Additional Voices (voice) |
| Daniel Mora als Additional Voices (voice) |
 | Eric Osmond als Additional Voices (voice) |
 | Noreen Reardon als Additional Voices (voice) |
 | Lee Shorten als Additional Voices (voice) |
 | Cree Summer als Additional Voices (voice) |
 | Nisa Ward als Additional Voices (voice) |
 | Nora Wyman als Additional Voices (voice) |
| Ed Skudder als Blue Shell Koopa (voice) (uncredited) |
| Chris Meledandri | Producer |
| Shigeru Miyamoto | Producer |
| Toyokazu Nonaka | Consulting Producer |
| Robert Taylor | Associate Producer |
| Aaron Horvath | Director |
| Michael Jelenic | Director |
| Ed Skudder | Head of Story |
| Brian Tyler | Original Music Composer |
| Koji Kondo | Music Consultant |
| Randy Thom | Sound Designer |
| Eric Osmond | Editor |
| Luis Velasco | Animation |
| Jeff Gabor | Animation |
| Christophe Delisle | Animation Director |
| Maxime Mary | Character Designer |
| Nicholas Olivieri | Character Designer |
| Philippe Tilikete | Character Designer |
| Sam Nielson | Character Designer |
| Daniel Fernandez-Casas | Character Designer |
| Tohru Patrick Awa | Character Designer |
| Fabien Polack | Co-Director |
| Pierre Leduc | Co-Director |
| Matthieu Gosselin | Art Direction |
| Ludovic Roz | Animation Director |
| Andree Hsiao-Chu Lin | Apprentice Sound Editor |
| Kelly Lake | Associate Producer |
| Alexandre Melquiond | Animation |
| Antoine Kinget | Animation |
| Christian Dan Bejarano | Animation |
| David Hill | Animation |
| Daniel Laurie | Supervising Sound Editor |
| Juan Peralta | Sound Re-Recording Mixer |
| Pete Horner | Sound Re-Recording Mixer |
| Laëtitia Grandjean | Production Manager |
| Guillaume Berthoumieu | Matte Painter |
| Thomas Revidon | Matte Painter |
| Cedric Launay | Lighting Supervisor |
| Damien Viatte | Lighting Supervisor |
| Jamey Scott | Sound Designer |
| Frédérick Alves-Cunha | Sculptor |
| Jérémie Droulers | Sculptor |
| Jasper Randall | Conductor |
| Christelle Balcon | Co-Producer |
| Julien Trouchet | Compositing Supervisor |
| Benjamin Morsberger | Creative Consultant |
| Natalie Watson | Creative Consultant |
| Nick Meledandri | Creative Consultant |
| Hadrien Vial | First Assistant Editor |
| Laurent de la Chapelle | In Memory Of |
| Brett Hoffman | Executive Producer |
| Bill Ryan | Executive Producer |
| Yusuke Beppu | Executive Producer |
| John R.A. Benson | Stereoscopic Supervisor |
| Guillaume Aretos | Production Design |
| Matthew Fogel | Writer |
| Tom Caulfield | Storyboard Artist |
| Jed Diffenderfer | Story Artist |
| Ed Skudder | Storyboard Artist |
| Ronni Brown | Foley Artist |
| Richard Duarte | Foley Mixer |
| Sean England | Foley Artist |
| Scott Guitteau | Sound Effects Editor |
| Devon Kelley | First Assistant Sound Editor |
| Leff Lefferts | Sound Effects Editor |
| Zach Martin | Foley Editor |
| E. Larry Oatfield | Foley Supervisor |
| Jacob Riehle | ADR Supervisor |
| Jana Vance | Foley Artist |
| Terah 'Bishop' Woodley II | Sound Re-Recording Assistant |
| Qianbaihui Yang | Sound Effects Editor |
| Jean-Marc Ariu | Modeling |
| Benoit Bernos | Modeling |
| Vincent Blanqué | VFX Lighting Artist |
| Christophe Brejon | VFX Lighting Artist |
| Eric Carme | Animation |
| Maria Castro Rodriguez | 3D Modeller |
| Ludovic Davidian | VFX Artist |
| Daniel de Benito | Compositing Artist |
| Mélissa Faucher | Software Engineer |
| Jade Gachet | Animation |
| Anne Getto | Visual Effects Supervisor |
| Corentin Gravend | Digital Compositor |
| Erwin Gresser | Compositing Lead |
| Michael Grund | VFX Artist |
| Kévin Herbrich | Modeling |
| Lucas Langou | VFX Artist |
| Marc Largier | Compositing Artist |
| Mathieu Le Meur | CG Supervisor |
| Baptiste Lemonnier | Modeling |
| Florian Magnin | VFX Artist |
| Astrid Massad | Modeling |
| Eric Mauhourat | Character Technical Supervisor |
| Marc Picco | Animation |
| Simon Popot | Animation |
| Kevin Razo | Digital Intermediate Colorist |
| Quentin Sauvinet | VFX Artist |
| Max Tourret | VFX Lighting Artist |
| Margaux Vaxelaire | Modeling |
| Christophe Vazquez | Visual Effects |
| Jocelyn Vincent | Visual Effects Supervisor |
| Mira Wendling | Animation |
| Guillaume Arrighi | Layout Supervisor |
| Loïc Baton | Animation |
| Julien Belloteau | Animation |
| Timo Berg | Animation |
| Adrien Braz | Animation |
| François Briantais | Animation |
| David Carrière | Animation |
| Jérôme Charton | Animation |
| Hugues Chauvin | VFX Artist |
| Augustin Clermont | Animation |
| Lucie Daniel | Animation |
| Hugues Daumail | VFX Artist |
| Jonathan del Val | Animation |
| Stéphane Dufournier | Modeling |
| Andy Dupont | VFX Artist |
| Marion Duvert | Animation |
| Bastien Fagoo | Animation |
| Hugo Falaize | Animation |
| Romain Faurie | Animation |
| Rita Figueira | Animation |
| Michaël Friederich | Animation |
| Simon Garriou | Animation |
| Stephane Hoarau | Pipeline Technical Director |
| John Harkins | VFX Artist |
| Elien Kellens | Animation |
| Pablo Iglesias | Animation |
| Patrick Le Bayon | VFX Artist |
| Clément Le Quéré | Animation |
| Vincent Lemaire | Animation |
| Rudi Loïal | Animation |
| Quentin Max | Animation |
| Scott McWhinnie | Animation |
| Olivier Montero | Animation |
| Giacomo Mora | Animation |
| Romain Peron | Animation |
| Michael Phy | Animation |
| Quentin Piq | Supervising Animator |
| Gaël Sabourin | Animation |
| Laura Servin | Animation |
| Jim Sweeting | Animation |
| Jackie Tarascio | Animation |
| Manuel Thomasser | VFX Artist |
| Luis Trebino | Animation |
| Clementine Tronel | Animation |
| Antoine Trouillet | Animation |
| Calvin Tsang | Story Artist |
| Timo Vermeulen | Animation |
| Maricruz Vállez | VFX Artist |
| Xavier Zahra | VFX Artist |
| Romana Reeve | Associate Editor |
| Patrick Clancey | Digital Intermediate Editor |
| Kristin Graham | Digital Intermediate Producer |
| Jason Hanel | Digital Intermediate Colorist |
| Michael Kannard | Imaging Science |
| Allie J. Mills | First Assistant Editor |
| Gregory Perler | Additional Editor |
| Ken Schretzmann | Additional Editor |
| Nathan Alexander | Music Arranger |
| Sara Andon | Musician |
| Rob Brophy | Musician |
| John Carey | Music Arranger |
| Sarah Casey | Music Coordinator |
| Rossano Galante | Orchestrator |
| Dylan Gentile | Vocals |
| William Kenneth Goldman | Vocals |
| Kelci Hahn | Vocals |
| DeLaney Harter | Musician |
| Andrew Kinney | Orchestrator |
| Jeff Kryka | Orchestrator |
| Michael Larco | Musician |
| Matthew Llewellyn | Music Editor |
| Max Lombardo | Music Arranger |
| Sara Mann | Vocals |
| Lindsay Patterson Abdou | Vocals |
| Dana Niu | Orchestrator |
| Mark Edward Smith | Vocals |
| Sarah Trevino | Music Arranger |
| Suzanne Waters | Vocals |
| Kenny Wood | Music Arranger |
| Josh Zimmerman | Music Arranger |
| Erin Katrina Hayes | Script Coordinator |
| Peter Rida Michail | Title Designer |
| Yōichi Kotabe | Thanks |
| Brice Colinet | Production Supervisor |
| David Pellé | CG Supervisor |
| Pierre Avon | Supervising Animator |
| Benjamin Faure | Supervising Animator |
| Benoit Guillaumot | Supervising Animator |
| Basile Heiderscheid | Supervising Animator |
| Adrien Kedochim | Supervising Animator |
| Mathieu Ménard | Supervising Animator |
| Nicolas Ménard | Supervising Animator |
| Aymeric Palermo | Supervising Animator |
| Ludovic Savonnière | Supervising Animator |
| Bruno Scheerens | Supervising Animator |
| Simon Pate | Effects Supervisor |
| Xavier Bec | Head of Research |
| Jeannine Berger | Post Production Producer |
| Tiffany Hillkurtz | Additional Editing |
| Katherine Ringgold | Additional Editing |
| Julian Smirke | Additional Editing |
| Andrew Walton | Additional Editing |
| Paul Mager | Set Designer |
| Vincent Massy | Set Designer |
| Long Ngo | Set Designer |
| Bertrand Piocelle | Set Designer |
| Gabriel Jolly-Monge | Set Designer |
| Philippe Diop | Set Designer |
| Frédéric Bontemps | Set Designer |
| Brian Tyler | Conductor |
| Dominique Boutonnat | Thanks |
| Vincent Florant | Thanks |
| Caroline Julliard-Mourgues | Thanks |
| Alex Hirsch | Thanks |
| Pauline Augrain | Thanks |
| Robert Elhai | Orchestrator |
| Brad Warnaar | Orchestrator |
| Jeff Toyne | Orchestrator |
| Ali Dee | Songs |
| Adam Yauch | Songs |
| Michael Diamond | Songs |
| Adam Horovitz | Songs |
| Anita Rachvelishvili | Songs |
| Giacomo Sagripanti | Songs |
| Bonnie Tyler | Songs |
| Jack Black | Songs |
| Koji Kondo | Music |
| Shinya Takahashi | Other |
| Yoshiaki Koizumi | Other |
| Takashi Tezuka | Other |
| Clément Griselain | Other |
| Lorenzo Veracini | Layout Supervisor |
| David Galante | Character Modelling Supervisor |
| Jean-Marc Imele | Other |
| Chloé Lesueur | Compositing Supervisor |
| Etienne Pêcheux | Supervising Technical Director |
| Milo Riccarand | Other |
| Shuntaro Furukawa | Other |
| Allie J. Mills | Other |
| Christophe Ducruet | Other |
| Silène Barraion | Other |
| Thomas Leroy | Other |
| Tifenn Philipott | Other |
| Laure Gauthier | Other |
| Matthieu Skrovec | Other |
| Marco Allard | Story Artist |
| David D. Au | Story Artist |
| Nima Azarba | Story Artist |
| Guy Bar'ely | Story Artist |
| Jerod Chirico | Story Artist |
| Bryan Cox | Story Artist |
| Eric Favela | Story Artist |
| Brandon Jeffords | Story Artist |
| Jeff Mednikow | Story Artist |
| AnChi Shen | Story Artist |
| Mark Walton | Story Artist |
| Tom Caulfield | Other |
| Maxime Delalande | Other |
| Jed Diffenderfer | Other |
| Serguei Kouchnerov | Other |
| James Krenzke | Other |
| Calvin Tsang | Other |
| Renan Porto | Colorist |
| Joël Corcia | Colorist |
| Sébastien Guichard | Colorist |
| Thomas Lépine | Colorist |
| Patrick Muyikens | Colorist |
| Marjolaine Dasnoy | Layout |
| Alex Angelis | Animation |
| Nicolas Loesner | Animation |
| Julien Madar | Animation |
| Haunui Maeta | Animation |
| Lucie Bossart | Animation |
| Géraud Martin | Animation |
| Quentin Bouillet | Animation |
| Pierre-Baptiste Marty | Animation |
| Renaud Buvat | Animation |
| Shinichi Ogawa | Thanks |
