 | Kate Beckinsale als Selene |
 | Theo James als David |
 | Tobias Menzies als Marius |
 | Lara Pulver als Semira |
 | Bradley James als Varga |
 | Peter Andersson als Vidar |
 | James Faulkner als Cassius |
 | Clementine Nicholson als Lena |
 | Daisy Head als Alexia |
 | Oliver Stark als Gregor |
 | Charles Dance als Thomas |
 | Zuzana Stivínová als Vampire Council #1 |
 | Brian Caspe als Vampire Council #2 |
 | Jan Nemejovský als Vampire Council #3 |
 | Sveta Driga als Amelia |
 | Dan Bradford als Lycan Pack Leader #1 |
 | David Bowles als Lycan Pack Leader #2 |
 | Tomas Fisher als Lycan Pack Leader #3 |
 | Rostislav Novák als Lycan Pack Leader #4 |
| Eva Larvoire als Tech Lycan |
 | Jiří N. Jelínek als Lycan Underling |
 | Marielle Tepper als Black Lycan Sidekick |
 | Jordan Haj als Lead Death Dealer |
 | Daniel Pietrucha als Death Dealer |
| Jakub Kropáček als Vampire Cadet #1 |
 | Sára Arnsteinová als Vampire Cadet #2 |
| Irena Morisakova als Cello Vampire #1 |
 | Terezie Kovalová als Cello Vampire #2 |
| Tomas Fingerland als Cello Vampire #3 |
| Jakub Mayer als Cello Vampire #4 |
| Denisa Kršková als Society Vampire #1 |
| Simona Simkova als Society Vampire #2 |
| Daniel Vojtech als Society Vampire #3 |
| Jared Doreck als Control Room Vampire |
| Lukas Vydrzal als Nordic Vampire #1 |
| Lukas Krampera als Nordic Vampire #2 |
| Adam Vosecek als Nordic Child #1 |
| Agatha Hofmanová als Nordic Child #2 |
| Anezka Nosková als Nordic Child #3 |
 | Alicia Vela-Bailey als Safehouse Lycan |
 | India Eisley als Eve (uncredited) |
 | Trent Garrett als Hybrid Michael (uncredited) |
| Martavious Gayles als Lycan (uncredited) |
| Miroslav Lhotka als Lycan in Tunnel (uncredited) |
| Richard William Romanov als Lycan (uncredited) |
 | Junes Zahdi als Lycan (uncredited) |
| Tom Rosenberg | Producer |
| Len Wiseman | Producer |
| Len Wiseman | Characters |
| Kevin Grevioux | Characters |
| Gary Lucchesi | Producer |
| Richard S. Wright | Producer |
| Richard S. Wright | Unit Production Manager |
| Skip Williamson | Executive Producer |
| Henry Winterstern | Executive Producer |
| James McQuaide | Executive Producer |
| James McQuaide | Visual Effects Supervisor |
| David Minkowski | Co-Producer |
| Matthew Stillman | Co-Producer |
| Martin Vačkář | Supervising Art Director |
| Ben Waisbren | Executive Producer |
| Karl Walter Lindenlaub | Director of Photography |
| Tony Lamberti | Sound Re-Recording Mixer |
| Peter Amundson | Editor |
| Eric Reid | Executive Producer |
| Michael Wandmacher | Music |
| Kyle Ward | Story |
| Cory Goodman | Screenplay |
| Cory Goodman | Story |
| Anna Foerster | Director |
| Anna Foerster | Executive Producer |
| Karel Fairaisl | Camera Operator |
| Ondrej Nekvasil | Production Design |
| Matt Mullins | Stunt Double |
| Bojana Nikitović | Costume Design |
| Ashleigh Dejon | Stunts |
| Ashleigh Dejon | Stunt Double |
| Paul Barry | First Assistant Director |
| Alicia Vela-Bailey | Stunts |
| Alicia Vela-Bailey | Stunt Double |
| David Kern | Producer |
| David Kern | Unit Production Manager |
| Danny McBride | Characters |
| Chris Diebold | Sound Effects Editor |
| Michal Holubec | Sound Recordist |
| Nevin Seus | Music Editor |
| Brian McNelis | Music Supervisor |
| Christopher S. Aud | Sound Designer |
| Suzanne Smith | Casting |
| Katerina van Gemundova | Art Department Coordinator |
| Jaromír Šedina | Camera Operator |
| Larry D. Horricks | Still Photographer |
| Michael Babcock | Supervising Sound Editor |
| Michael Babcock | Sound Re-Recording Mixer |
| Jeff Sawyer | Sound Effects Editor |
| Vanessa Lapato | Dialogue Editor |
| Michael Landgrebe | Visual Effects Supervisor |
| Laura Windows | Casting Associate |
| Lauren Hadaway | ADR & Dubbing |
| Paul Janssen | CG Supervisor |
| Alex Kornreich | Additional Camera |
| Holger Hummel | Visual Effects Producer |
| Matt Weaver | Animation |
| Maya Kvetny | Casting |
| Jamie Hallett | Visual Effects Supervisor |
| Šárka Zázvorková | Costume Supervisor |
| Todd Schneider | Stunt Coordinator |
| Bryce Tibbey | Art Direction |
| Eric Craig | Music Supervisor |
| Natasha Lees | Makeup Artist |
| Davina Lamont | Makeup Designer |
| Jackie Shenoo | Co-Producer |
| Julie Hutchinson | Casting |
| Jaime Leigh McIntosh | Hairstylist |
| Siggy Ferstl | Digital Intermediate |
| Jared Krichevsky | Creature Design |
| Veronika Metzova | Key Costumer |
| Michael Gilbert | Visual Effects Editor |
| Nina Moravcova | Set Costumer |
| Jirí Trier | Set Costumer |
| Dan Balzer | First Assistant Camera |
| Gary Winter | Gaffer |
| Justin Brunett | Animation |
| Ngan Chung | Animation |
| Meghan Grube | Animation |
| Fraser Page | Animation |
| Jaco Pretorius | Animation |
| Erica Robinson | Animation |
| Andrew Zink | CG Supervisor |
| Josh Simmonds | VFX Supervisor |
| Eva Peschkes | Visual Effects Coordinator |
| Ludek Vomacka | Visual Effects Coordinator |
| Chase Aston | Makeup Artist |
| Alexandre Cancado | 2D Supervisor |
| Katie Eischen | Stunts |
| Monika Hlavatá | Script Supervisor |
| Petr Richter | Property Master |
| Bart de Haan | Russian Arm Operator |
| Luka Antonić | Costume Coordinator |
| Megan Shank | Second Assistant Director |
| Martin David | Stunts |
| Marie Svecova | Stunts |
