 | Kate Beckinsale als Selene |
 | Scott Speedman als Michael Corvin |
 | Tony Curran als Marcus Corvinus |
 | Shane Brolly als Kraven |
 | Derek Jacobi als Alexander Corvinus |
 | Bill Nighy als Viktor |
 | Steven Mackintosh als Andreas Tanis |
 | Zita Görög als Amelia |
 | Brian Steele als William Corvinus |
 | John Mann als Samuel |
 | Scott McElroy als Soren |
 | Michael Sheen als Lucian |
 | Sophia Myles als Erika |
 | Richard Cetrone als Pierce |
| Mike Mukatis als Taylor |
 | Raoul Ganeev als Truck Driver |
 | Andrew Kavadas als Selene's Father |
| Kayla Levins als Selene's Sister |
| Attila Lovaghy als Tavern Cop #1 |
| Monica Hamburg als Newscaster |
| Travis Webster als Cleaner |
 | Kaja Gjesdal als Tanis Vamp #2 |
 | Lily Mo Sheen als Young Selene |
 | Adrian Hough als Pilot |
 | Michasha Armstrong als Gunner |
 | Krsy Fox als Tanis Vamp #1 |
| Julius Chapple als French Cleaner Aide |
| Tom Rosenberg | Producer |
| Scott Martin Gershin | Sound Designer |
| Len Wiseman | Director |
| Len Wiseman | Executive Producer |
| Len Wiseman | Characters |
| Len Wiseman | Story |
| Kevin Grevioux | Characters |
| Kevin Grevioux | Co-Producer |
| Gary Lucchesi | Producer |
| Richard S. Wright | Producer |
| Skip Williamson | Executive Producer |
| Henry Winterstern | Executive Producer |
| Terry McKay | Executive Producer |
| James McQuaide | Executive Producer |
| Wendy Partridge | Costume Design |
| Patrick Tatopoulos | Production Design |
| Deborah Aquila | Casting |
| Marco Beltrami | Original Music Composer |
| Marco Beltrami | Conductor |
| Nicolas De Toth | Editor |
| David Leitch | Stunts |
| David Coatsworth | Producer |
| David Coatsworth | Unit Production Manager |
| Simon Duggan | Director of Photography |
| Chris August | Art Direction |
| Lin MacDonald | Set Decoration |
| Bryan Bowen | Sound Effects Editor |
| Chris Palermo | Stunts |
| Jennifer L. Smith | Casting Associate |
| Scott Cozens | Loader |
| Aaron Meziere | Key Set Production Assistant |
| Clint Carleton | Stunts |
| Rosalina Da Silva | Key Makeup Artist |
| Monique Ganderton | Stunts |
| Stephen Jackson | Chief Lighting Technician |
| Brad Martin | Stunt Coordinator |
| Brad Martin | Second Unit Director |
| Kirk Caouette | Stunt Double |
| Ron Bedrosian | ADR Mixer |
| James Moriana | Foley Artist |
| Hollywood Heard | Best Boy Electric |
| Tricia Wood | Casting |
| Doug Chapman | Stunt Double |
| Larkin MacKenzie-Ast | Casting Assistant |
| Sam Harris | First Assistant Director |
| Mike Fields | Makeup Effects |
| Owen Walstrom | Stunt Driver |
| Jim Filippone | Pilot |
| Danny McBride | Screenplay |
| Danny McBride | Executive Producer |
| Danny McBride | Characters |
| Danny McBride | Story |
| Jana MacDonald | Assistant Costume Designer |
| Paolo G. Venturi | Assistant Art Director |
| Vincent Cirelli | CG Supervisor |
| Dan Sissons | Property Master |
| Jaydene Maryk | Art Department Assistant |
| Lloyd Lee Barnett | Visual Effects Supervisor |
| Brian McNelis | Music Supervisor |
| John T. Cucci | Foley Mixer |
| Allison Klein | Executive Assistant |
| Brian Shell | Construction Coordinator |
| David Crone | Camera Operator |
| David Crone | Steadicam Operator |
| Joel Whist | Special Effects Coordinator |
| Blue Angus | Transportation Coordinator |
| Larry Kemp | ADR Supervisor |
| Daniel S. Irwin | Dialogue Editor |
| John Burke | Set Designer |
| Douglas Curran | Still Photographer |
| Nicole Randall | Stunt Double |
| Artin Matousian | Systems Administrators & Support |
| Sean M. Harding | First Assistant Camera |
| Trevor Ward | Dolby Consultant |
| Lee Anne Muldoon | Unit Publicist |
| Michael Toby | Production Illustrator |
| Rick Rasmussen | Picture Car Coordinator |
| Merribelle Anderson | Hairstylist |
| Des Carey | Digital Intermediate Producer |
| Daniel Scarcello | Sculptor |
| Gary Oldroyd | Post Production Coordinator |
| Denise Okimoto | Music Editor |
| David Heinz | Visual Effects Editor |
| Bridget Allen | Production Supervisor |
| Thom MacIntyre | Key Hair Stylist |
| Terry Calhoun | Assistant Chief Lighting Technician |
| Brad Alexander | Lighting Artist |
| Franco Pante | Assistant Editor |
| Franziska Keller | Art Department Coordinator |
| Kee Kee Buckley | Thanks |
| James Andrykowski | First Assistant Editor |
| Stephen R. Sheridan | Color Timer |
| Derek Ledbetter | Visual Effects |
| Patrick Cusack | Assistant Sound Editor |
| Petra Bergholz | Seamstress |
| David Husby | Sound Mixer |
| Keith Splinter Davis | Stunts |
| Charles O'Shea | Boom Operator |
| Siggy Ferstl | Digital Intermediate Colorist |
| Ken Decker | Generator Operator |
| Nick Diomis | Transportation Captain |
| Andy Chung | Concept Artist |
| Antonio Germinario | Assistant Property Master |
| Robert L. Benun | Thanks |
| Brian Bero | Armorer |
| Miguel Ortega | Modeling |
| Tim Webb | Sound Recordist |
| Misha Bukowski | Third Assistant Director |
| Mark Moretti | Storyboard Artist |
| Kim Olsen | First Company Grip |
| Dan Akers | Digital Compositors |
| H. Christopher Hunter | Dolly Grip |
| Niles Harrison | Visual Effects Coordinator |
| Corry Glass | Stunts |
| Monica Sandstede | Story Editor |
| Dan Cervin | Special Effects Assistant |
| Artaya Boonsoong | Technical Supervisor |
| Justin Johnson | Compositing Supervisor |
| Jody Chow | Assistant Production Coordinator |
| Teresa A. Meyer | Makeup Artist |
| Wayne 'Woody' Cone | Key Grip |
| Paul Arnel | Grip |
| Greyson R. Wolf | Prosthetic Makeup Artist |
| John T. Boe | Set Dressing Artist |
| Dale Allen | Carpenter |
| Lee McNish | Chef |
| Ildiko Barritt | Craft Service |
| Dwayne Deverill | Driver |
| Casey Dexter | Post Production Assistant |
| Mark Hoeppner | Security |
| Renée George | Electrician |
| Rick Dean | Lighting Technician |
| Ralph Guttman | Rigging Grip |
| Kendrie Upton | Location Manager |
| Julia Bogdan-Rollo | Production Accountant |
| Craig Dumont | Production Coordinator |
| Elizabeth Hill | Production Manager |
| Glen Suhy | 3D Artist |
| Cameron Gray | Animation Supervisor |
| Phil Notaro | Special Effects Supervisor |
| Alex Brownell | Visual Effects Producer |
| Ken Brooker | Assistant Location Manager |
| Julio Carmona | ADR Recordist |
| Stephanie Boeke | Extras Casting |
| Mark Gordon | Second Assistant Camera |
| Chris Bignell | Special Effects Technician |
| Marcine Peter | Contact Lens Technician |
| Brent Bennett | Set Buyer |
| Gareth Farfan | Second Company Grip |
| Leon Serginson | Assistant Camera |
| Debbie Humphreys | Wardrobe Supervisor |
| Darren Robertson | Playback Coordinator |
| Steven Flegel | Second Assistant Production Coordinator |
| Maureen Carey | Construction Buyer |
| Michele Lee | First Assistant Accountant |
| Ishi Dinim | Camera Trainee |
| Jeff Barnes | Production Executive |
| Dinesh Gill | Property Buyer |
| Patty Hunter | Costume Set Supervisor |
| Kia Porter | Dresser |
| Donald Campbell | VFX Artist |
| James Kantola | Best Boy Grip |
| Terry Shigemitsu | CG Artist |
| Ralph Denson | General Manager |
| Wendy Klein | Matte Painter |
| Ryan Garcia | Production Assistant |
| Caroline Field | Stunts |
