 | Yo Oizumi als Professor Layton (voice) |
 | Maki Horikita als Luke Triton (voice) |
 | Saki Aibu als Emmy Altava (voice) |
 | Nana Mizuki als Janice Quatlane (voice) |
 | Atsuro Watabe als Jean Descole (voice) |
 | Hochu Otsuka als Inspector Clamp Grosky (voice) |
 | Iemasa Kayumi als Oswald Whistler (voice) |
 | Fumiko Orikasa als Melina Whistler (voice) |
 | Kikuko Inoue als Celia Raidley (voice) |
 | Sumire Morohoshi als Nina (voice) |
 | Shozo Iizuka als Curtis O'Donnell (voice) |
 | Kenta Miyake als Marco Brock (voice) |
 | Megumi Toyoguchi als Amelia Ruth (voice) |
 | Jouji Nakata als Frederick Bargland (voice) |
 | LiLiCo als Annie Dretche (voice) |
 | Koichi Yamadera als Pierre Starbuck (voice) |
 | Minoru Inaba als Don Paolo (voice) |
 | Shirō Saitō als Inspector Chelmey (voice) |
 | Mamiko Noto als Flora Reinhold (voice) |
 | Rokuro Naya als Dr. Andrew Schrader (voice) |
| Masakazu Hashimoto | Director |
| Tomohito Nishiura | Original Music Composer |
| Yumenosuke Tokuda | Key Animation |
| Nobutoshi Ogura | Key Animation |
| Hiroyuki Horiuchi | Key Animation |
| Hiroshi Shimizu | Key Animation |
| Reiko Okamura | Key Animation |
| Noboru Sugimitsu | Key Animation |
| Mitsutoshi Kubo | Key Animation |
| Toshihisa Kaiya | Key Animation |
| Noboru Sugimitsu | Animation Director |
| Hiroyuki Horiuchi | Animation Director |
| Norihiro Matsubara | Animation Director |
| Sayuri Ichiishi | Animation Director |
| Shinichi Yoshino | Animation Director |
| Kayoko Nabeta | Animation Director |
| Hideki Takahashi | Animation Director |
| Kosuke Kawatsura | Animation Director |
| Kosuke Kawatsura | Key Animation |
| Kayoko Nabeta | Key Animation |
| Fumihiko Suganuma | Key Animation |
| Asami Hayakawa | Key Animation |
| Yasuo Fujii | Key Animation |
| Asuka Kojima | Key Animation |
| Kento Toya | Key Animation |
| Yurie Oohigashi | Key Animation |
| Miho Daidouji | Key Animation |
| Katsumi Ikeda | Key Animation |
| Takao Maki | Key Animation |
| Tsuyoshi Yoshioka | Key Animation |
| Akiharu Ishii | Key Animation |
| Kenji Irie | Key Animation |
| Miyuki Ueda | Key Animation |
| Akira Kojima | Key Animation |
| Naoko Ise | Key Animation |
| Aya Nakanishi | Key Animation |
| Takashi Tsuda | Key Animation |
| Atsushi Ogata | Key Animation |
| Yasue Ohno | Key Animation |
| Tomohiro Koyama | Key Animation |
| Tomoki Ogawa | Key Animation |
| Junpei Fukuchi | Key Animation |
| Hosato Shima | Key Animation |
| Misaki Suzuki | Key Animation |
| Hiromi Makino | Key Animation |
| Yasukazu Shoji | Key Animation |
| Koji Fukasawa | Key Animation |
| Hidetoshi Owashi | Key Animation |
| Tadaaki Miyata | Key Animation |
| Yuuko Yoshida | Key Animation |
| Mayumi Miyaoka | Key Animation |
| Aiko Kasumi | Key Animation |
| Yuko Iwaoka | Key Animation |
| Takahiro Kuroishi | Key Animation |
| Masakazu Kubo | Executive Producer |
| Kazuya Hamana | Executive Producer |
| Naoya Fujimaki | Co-Executive Producer |
| Shuji Abe | Co-Executive Producer |
| Masakazu Hashimoto | Storyboard Artist |
| Hiroyuki Imaishi | Storyboard Artist |
| Joji Shimura | Storyboard Artist |
| Junji Nishimura | Storyboard Artist |
| Souichi Masui | Storyboard Artist |
| Naoto Yamatani | Sound Effects |
| Sachiko Nishi | Sound Effects |
| Kosuke Ogata | Sound Effects |
| Tomokazu Mii | Sound Effects |
| Takuya Hasegawa | Sound Effects |
| Akiko Muto | Sound Effects |
| Shizuo Kurahashi | Sound Effects |
| Masafumi Mima | Sound Director |
| Yusuke Takeda | Art Direction |
| Toshio Henmi | Editor |
| Riko Shinohara | Art Direction |
| Noboru Sugimitsu | Character Designer |
| Noboru Sugimitsu | Supervising Animation Director |
| Aya Matsui | Screenplay |
| Toshiaki Okuno | Producer |
| Kenji Horikawa | Producer |
| Arimasa Okada | Producer |
| Akihiro Yamauchi | Producer |
| Tsuneyoshi Saito | Main Title Theme Composer |
| Hiroshi Aso | Co-Producer |
| Toshihiro Kitta | Co-Producer |
| Koji Goromaru | Co-Producer |
| Nobuhiro Kikuchi | Co-Producer |
| Madoka Hosoya | Co-Producer |
| Koichi Sato | Key Animation |
