 | Jason Alexander als Eugene (voice) |
 | Tim Curry als Doctor Dolittle (voice) |
 | Jane Seymour als Polynesia (voice) |
 | Tom Kenny als Gordo / Chomps / Jip (voice) |
 | Maddie Taylor als Brainy Ape / Tango (voice) |
 | André Sogliuzzo als Crocodile Leader / Ramsey (voice) |
 | Dannah Feinglass Phirman als Dab-Dab / Mrs. Dolittle / Zen Zebra (voice) |
 | Mackenzie Mason als Little Bear / Little Kitty (voice) |
 | Stevie Vallance als Little Bunny / Little Puppy / Little Rhino (voice) |
 | Jessica DiCicco als Louise / Ping-Ping (voice) |
| Jenn Reese als Ms. Ping / Young Doctor Dolittle (voice) |
 | Marc Graue als Yellephant (voice) |
| Rinat Gazizov | Director |
| Phil Harnage | Writer |
| Tom Ruegger | Writer |
| Lindsay Schulz | Writer |
| Lindsay Schulz | Producer |
| Christopher Lofting | Executive Producer |
| Steve Waterman | Executive Producer |
| Ed Hunsaker | Supervising Producer |
| Cooper Waterman | Co-Producer |
| Kevin O'Donnell | Co-Producer |
| Rinat Gazizov | Character Designer |
| Rinat Gazizov | Storyboard Artist |
| Frank Rocco | Additional Storyboarding |
| Karina Gazizov | Additional Storyboarding |
| Nelson Brann | Editor |
| Jonelle Perry | Associate Producer |
| David Burke | Original Music Composer |
| David Burke | Vocal Coach |
| Andrew Pagana | Vocal Coach |
| Stevie Vallance | Vocal Coach |
| Ericka Martinez | Production Office Coordinator |
| Ruth Jennifer Erskine | Executive Producer's Assistant |
| Sarah Kim | Production Accountant |
| Nicholas Arnold | Legal Services |
| Jeffrey Kranzdorf | Legal Services |
| Phil Mendez | Character Designer |
| Hugh Lofting | Characters |
| Eddie Fitzgerald | Storyboard Artist |
