 | Paul Reubens als Pee-wee Herman |
 | Phil Hartman als Kap'n Karl |
 | Lynne Marie Stewart als Miss Yvonne |
 | John Paragon als Jambi the Genie |
 | Edie McClurg als Hermit Hattie |
 | John Moody als Mailman Mike |
 | Tito Larriva als Hammy |
 | Nicole Panter als Susan |
| Monica Ganas als Mrs. Jelly Donut |
| Brian Seff als Mr. Jelly Donut |
| Joan Leizman als Joan |
| Marty Callner | Director |
| Paul Reubens | Writer |
| Phil Hartman | Writer |
| Edie McClurg | Writer |
| John Paragon | Writer |
| Bill Steinkellner | Writer |
| Paul Reubens | Executive Producer |
| Dawna Kaufmann | Co-Producer |
| Gregory Sills | Associate Producer |
| Gary Panter | Production Design |
| Jay Condom | Music Arranger |
| Jay Condom | Music |
| Greg Brunton | Lighting Director |
| Patrick Aumont | Production Coordinator |
| Betsy Heimann | Production Coordinator |
| Leslie Williams | Helping Hand |
| Dwight Jackson | Art Direction |
| Tommy Cole | Makeup Artist |
| Joe Epperson | Camera Operator |
| Larry Heider | Camera Operator |
| Bobby Keyes | Camera Operator |
| Dave Levisohn | Camera Operator |
| Hector Ramirez | Camera Operator |
| Rick Nelson | Special Effects |
| Paul Reubens | Stage Director |
| Lynne Marie Stewart | Writer |
| John Moody | Writer |
| Tito Larriva | Writer |
| Nicole Panter | Writer |
| Monica Ganas | Writer |
| Brian Seff | Writer |
| Joan Leizman | Writer |
| Barbara Ling | Lighting Design |
| Betsy Heimann | Costume Design |
