 | Kate Beckinsale als Selene |
 | Stephen Rea als Dr. Jacob Lane |
 | Michael Ealy als Detective Sebastian |
 | Theo James als David |
 | India Eisley als Eve |
 | Charles Dance als Thomas |
 | Sandrine Holt als Lida |
 | Sanny van Heteren als British newscaster |
 | Jacob Blair als Kolb (Officer) |
 | Robert Lawrenson als Waterfront Cop |
 | Kurt Max Runte als Leader of the Troop |
 | Marvin Duerkholz als Lestroy |
| Ron Wear als Jack Fletcher |
 | Richard Cetrone als Lycan 1 |
 | Dan Payne als Lycan 2 |
 | Mark Gibbon als Announcing Guard |
 | Benita Ha als Surgical Nurse |
| Tyler McClendon als Scientist |
 | Panou als Old City Cop #1 |
 | Ian Rozylo als Old City Cop #2 |
 | Kristen Holden-Ried als Quint Lane |
 | John Innes als Medical Supervisor |
 | Lee Majdoub als Desk Guard |
 | Catlin Adams als Olivia |
 | Adam Greydon Reid als Med Tech |
 | Christian Tessier als Security Guard #1 |
 | Wes Bentley als Antigen Scientist (uncredited) |
 | Scott Speedman als Michael (archive footage) (uncredited) |
| Sanny van Heteren als BBC Announcer (uncredited) |
| Joe Censoplano als Cadillac Driver (uncredited) |
| Preston Cook als Antigen Surgeon (uncredited) |
| Tom Rosenberg | Producer |
| Paul Haslinger | Original Music Composer |
| Len Wiseman | Screenplay |
| Len Wiseman | Producer |
| Len Wiseman | Characters |
| Len Wiseman | Story |
| Kevin Grevioux | Characters |
| Gary Lucchesi | Producer |
| Richard S. Wright | Producer |
| Skip Williamson | Executive Producer |
| Henry Winterstern | Executive Producer |
| James McQuaide | Executive Producer |
| James McQuaide | Visual Effects Supervisor |
| Deborah Aquila | Casting |
| Monique Prudhomme | Costume Design |
| David Coatsworth | Executive Producer |
| Claude Paré | Production Design |
| Scott Kevan | Director of Photography |
| J. Michael Straczynski | Screenplay |
| Eric Reid | Executive Producer |
| Allison Burnett | Screenplay |
| Kimberly Chiang | Stunts |
| Duane Dickinson | Stunts |
| Krista Bell | Stunts |
| Shane Vieau | Set Decoration |
| Rosalina Da Silva | Makeup Department Head |
| Måns Mårlind | Director |
| Björn Stein | Director |
| J.J. Perry | Stunt Coordinator |
| Lauro Chartrand | Stunts |
| John Hlavin | Screenplay |
| John Hlavin | Story |
| Brad Martin | Stunt Coordinator |
| Gary Myers | Art Direction |
| Jeff McEvoy | Editor |
| Brett Armstrong | Stunts |
| Tricia Wood | Casting |
| Lis Kern | Post Production Supervisor |
| Alicia Vela-Bailey | Stunt Double |
| David Kern | Executive Producer |
| Owen Walstrom | Stunt Driver |
| Danny McBride | Characters |
| Janene Carleton | Stunts |
| Martina Javorova | Art Direction |
| Christine Wilson | Script Supervisor |
| Nicholas Podbrey | Makeup Effects |
| Earl A. Hibbert | Visual Effects Editor |
| Seung Hoo Ihm | Animation |
| Rochelle Okoye | Stunts |
| Stewart Whelan | First Assistant Camera |
| Atlin Mitchell | Stunts |
| Atlin Mitchell | Stunt Double |
| Jeffrey G. Barnett | Stunts |
| Sabine Varnes | Stunts |
| Corry Glass | Stunt Driver |
| Alicia Turner | Stunts |
| Frédéric Côté | Senior Animator |
| Katherine Fraser | Stunts |
