 | Reese Witherspoon als Susan Murphy / Ginormica (voice) |
 | Seth Rogen als B.O.B. (voice) |
 | Hugh Laurie als Dr. Cockroach Ph.D. (voice) |
 | Rainn Wilson als Gallaxhar (voice) |
 | Will Arnett als The Missing Link (voice) |
 | Kiefer Sutherland als General W.R. Monger (voice) |
 | Paul Rudd als Derek Dietl (voice) |
 | Stephen Colbert als President Hathaway (voice) |
 | Julie White als Wendy Murphy (voice) |
 | Jeffrey Tambor als Carl Murphy (voice) |
 | Amy Poehler als Computer (voice) |
 | Ed Helms als News Reporter (voice) |
 | Renée Zellweger als Katie (voice) |
 | Sean Bishop als Private Bullhorn / Helicopter Pilot / Advisor Ortega (voice) |
| Rich Dietl als Commander / Advisor Smith (voice) |
 | Rob Letterman als Secret Service Man #1 / Lieutenant (voice) |
 | Tom McGrath als Wilson (voice) |
 | Chris Miller als Advisor Cole / Army Commander Jones (voice) |
 | Mike Mitchell als Advisor Wedgie (voice) |
 | Kent Osborne als Technician Jerry (voice) |
 | Latifa Ouaou als Advisor Ouaou / Mrs. Ronson / Female Advisor #2 / Panicked Guest (voice) |
 | Geoffrey Pomeroy als Soldier #4 / Pilot / Soldier #3 / News Technician (voice) |
 | David P. Smith als Soldier #1 / Advisor Jackson (voice) |
| Lisa Stewart als Bridesmaid Candy (voice) |
 | Conrad Vernon als Advisor Hawk / Advisor Dither / Minister / Secret Service Man #2 / Mama Dietl (voice) |
 | John Krasinski als Cuthbert (voice) |
 | Stephen Kearin als Technician Bee (voice) |
| Rob Letterman | Director |
| Maya Forbes | Screenplay |
| Wallace Wolodarsky | Screenplay |
| Glenn Berger | Screenplay |
| Rob Letterman | Screenplay |
| Jonathan Aibel | Screenplay |
| Conrad Vernon | Director |
| James Baxter | Animation |
| Steve Cunningham | Animation |
| John Bruno | Thanks |
| Ken Bielenberg | Visual Effects Supervisor |
| David James | Production Design |
| Anna Behlmer | Sound Mixer |
| Ethan van der Ryn | Supervising Sound Editor |
| Michael Isaak | Art Direction |
| Erik Aadahl | Supervising Sound Editor |
| Scott Wills | Art Direction |
| Erika Dapkewicz | Editor |
| Damon O'Beirne | Cinematography |
| Andy Nelson | Sound Mixer |
| Henry Jackman | Original Music Composer |
| Joyce Arrastia | Editor |
| Craig Kellman | Character Designer |
| Lisa Stewart | Producer |
| Jill Hopper | Co-Producer |
| Latifa Ouaou | Co-Producer |
| Hans Zimmer | Executive Music Producer |
| Daniela Mazzucato | Production Manager |
| Leslee Feldman | Casting |
| Joe Monteleone | First Assistant Editor |
| David Yanover | Post Production Supervisor |
| Kate Crossley | Post Production Supervisor |
| Susan Dudeck | ADR Supervisor |
| Wayne Lemmer | ADR Editor |
| John Marquis | Sound Effects Editor |
| P.K. Hooker | Sound Effects Editor |
| Jonathan Klein | Foley Editor |
| Dan O'Connell | Foley Artist |
| John T. Cucci | Foley Artist |
| James Ashwill | Foley Mixer |
| Richard Duarte | Foley Mixer |
| Thomas J. O'Connell | ADR Mixer |
| Rick Canelli | ADR Recordist |
| Gene Goins | Projection |
| Jim Passon | Color Timer |
| Adam Milo Smalley | Music Editor |
| Philip Cohen | Production Supervisor |
| Rich Dietl | First Assistant Editor |
